Invitrogen

RAB7 Polyclonal Antibody

Name: RAB7 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-23138

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3 References

View all (15) RAB7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Advanced Verification (1)

RAB7 Antibody in Immunocytochemistry (ICC/IF)

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RAB7 Antibody (PA5-23138) in ICC/IF

Immunocytochemistry analysis of RAB7 in NIH3T3 cells. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-23138) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue).... View More

RAB7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RAB7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAB7 siRNA and reduction of signal was observed in Western Blot using RAB7 Polyclonal Antibody (Product # PA5-23138). {KD}

Immunogen image

Immunogen Image RAB7 Polyclonal Antibody

Product Details

PA5-23138

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

2-5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A portion of amino acids 90-140 of human Rab7 protein.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2540665

Target Information

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein Rab-7a

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Gene Aliases: PRO2706; RAB7; RAB7A

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UniProt ID: (Human) P51149, (Mouse) P51150

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Entrez Gene ID: (Human) 7879, (Mouse) 19349

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Function(s)

GTPase activity protein binding GTP binding GDP binding Rac GTPase binding nucleotide binding

Process(es)

protein targeting to lysosome transport intracellular protein transport epidermal growth factor catabolic process small GTPase mediated signal transduction endosome to lysosome transport protein transport viral release from host cell protein to membrane docking Rab protein signal transduction retrograde transport, endosome to Golgi early endosome to late endosome transport bone resorption positive regulation of protein catabolic process positive regulation of viral process phagosome acidification phagosome-lysosome fusion positive regulation of exosomal secretion regulation of autophagosome assembly endocytosis antigen processing and presentation of exogenous peptide antigen via MHC class II lipophagy phagosome maturation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB7 Polyclonal Antibody

RAB7 Antibody (PA5-23138) in ICC/IF

Product Details

PA5-23138

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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