Invitrogen

RAB3A Monoclonal Antibody (OTI1G3)

Name: RAB3A Monoclonal Antibody (OTI1G3)
Brand: Invitrogen
SKU: MA5-27162

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1 Reference

View all (27) RAB3a antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (12)
Advanced Verification (6)

RAB3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 18

RAB3A Antibody (MA5-27162) in IHC (P)

Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a RAB3A monoclonal antibody (Product # MA5-27162) at a dilution of 1:150.

Product Image Gallery

RAB3A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of RAB3A was observed in Rat Brain and Cerebellum in comparison to Rat Skeletal muscle and Liver using RAB3A Monoclonal Antibody (OTI1G3) (Product # MA5-27162) in western blot. (10.1128/MCB.22.6.1858-1867.2002). {RE}

Immunogen image

Immunogen Image RAB3A Monoclonal Antibody (OTI1G3)

Product Details

MA5-27162

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:1,2000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1G3

Immunogen

Full length human recombinant protein of RAB3A produced in E.coli

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2725178

Target Information

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. Specifically, Rab 3s are thought to play a key role in presynaptic vesicle trafficking and priming.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C18A3.6; RAS-associated protein RAB3A; Ras-related protein Rab-3A; Ras-related small GTP binding protein 3A

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Gene Aliases: RAB3; RAB3A

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UniProt ID: (Human) P20336, (Rat) P63012, (Mouse) P63011

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Entrez Gene ID: (Human) 5864, (Rat) 25531, (Mouse) 19339

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Function(s)

ATPase activator activity GTPase activity protein binding GTP binding protein C-terminus binding myosin V binding GTPase binding ATPase binding GDP binding nucleotide binding

Process(es)

respiratory system process intracellular protein transport vesicle docking involved in exocytosis mitochondrion organization neuromuscular synaptic transmission axonogenesis metabolic process protein secretion post-embryonic development synaptic vesicle exocytosis synaptic vesicle maturation regulation of exocytosis lung development regulation of synaptic vesicle fusion to presynaptic membrane Rab protein signal transduction positive regulation of ATPase activity synaptic vesicle recycling regulation of short-term neuronal synaptic plasticity maintenance of presynaptic active zone structure sensory perception of touch response to electrical stimulus protein localization to plasma membrane positive regulation of regulated secretory pathway transport exocytosis small GTPase mediated signal transduction protein transport synaptic vesicle transport evoked neurotransmitter secretion regulation of long-term synaptic potentiation neurotransmitter secretion glutamate secretion constitutive secretory pathway positive regulation of exocytosis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB3A Monoclonal Antibody (OTI1G3)

RAB3A Antibody (MA5-27162) in IHC (P)

Product Details

MA5-27162

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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