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  • Primary Antibodies ›
  • RAB1A Antibodies

Invitrogen

RAB1A Polyclonal Antibody

View all (16) RAB1A antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite RAB1A Polyclonal Antibody

  • Antibody Testing Data (7)
RAB1A Antibody in Immunocytochemistry (ICC/IF)
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RAB1A Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 7

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RAB1A Antibody (PA5-27421) in ICC/IF

Knockout of RAF1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR627776_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of RAF1 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa Cas9 (Lane 2) and HeLa RAF1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by i... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RAB1A Antibody in Immunocytochemistry (ICC/IF)
RAB1A Antibody in Western Blot (WB)
RAB1A Antibody in Western Blot (WB)
RAB1A Antibody in Western Blot (WB)
RAB1A Antibody in Western Blot (WB)
RAB1A Antibody in Western Blot (WB)
RAB1A Antibody in Western Blot (WB)

Product Details

PA5-27421

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rab 1A. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544897

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, rat brain, Rab 1A tansfected 293T cell.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Dog (100%), Pig (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). This gene is co-transcribed with the U68 snoRNA, located within the third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZP564B163; GTP binding protein Rab1a; Rab GTPase YPT1 homolog; RAB1, member RAS oncogene family; Ras-related protein Rab-1A; ras-related YPT1 protein; YPT1-related protein

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Gene Aliases: Ac2-048; Gtbp; mKIAA3012; Rab-1; RAB1; RAB1A; Rab1r; YPT1

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UniProt ID: (Human) P62820, (Mouse) P62821, (Rat) Q6NYB7

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Entrez Gene ID: (Human) 5861, (Mouse) 19324, (Rat) 81754

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Function(s)
GTPase activity protein binding GTP binding cadherin binding involved in cell-cell adhesion nucleotide binding GDP binding
Process(es)
autophagosome assembly intracellular protein transport ER to Golgi vesicle-mediated transport endocytosis autophagy cell migration virion assembly growth hormone secretion Rab protein signal transduction defense response to bacterium vesicle transport along microtubule interleukin-8 secretion establishment of endothelial intestinal barrier positive regulation of glycoprotein metabolic process regulation of autophagosome assembly retrograde vesicle-mediated transport, Golgi to ER Golgi organization small GTPase mediated signal transduction vesicle-mediated transport melanosome transport substrate adhesion-dependent cell spreading cilium assembly COPII vesicle coating cargo loading into COPII-coated vesicle cell-cell adhesion transport protein transport
It has to be done as per old AB suggested Products section.
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