Invitrogen

Prohibitin Monoclonal Antibody (II-14-10)

Advanced Verification
7 Published Figures
59 References
View all (53) Prohibitin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-12858

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1.0 µg/mL
View 44 publications 44 publications

Immunohistochemistry (IHC)

-
View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL
View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-
View 3 publications 3 publications

Flow Cytometry (Flow)

-
View 2 publications 2 publications

Immunoprecipitation (IP)

-
View 1 publication 1 publication

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Chicken, Human, Mouse, Non-human primate, Pig, Rat

Published species

Avian, Human, Mouse, Pig, Rat, Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

II-14-10

Immunogen

Purified recombinant rat prohibitin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980219

Product Specific Information

MA5-12858 targets Prohibitin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with Chicken, Human, mouse, Porcine, and Rat samples.

The MA5-12858 immunogen is purified recombinant rat prohibitin protein.

Target Information

Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1

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Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1

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UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779

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Entrez Gene ID: (Human) 5245, (Chicken) 419980, (Mouse) 18673, (Rat) 25344

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding complement component C3a binding complement component C3b binding protein binding protein C-terminus binding enzyme binding proteinase activated receptor binding histone deacetylase binding transcription regulatory region DNA binding molecular_function
Process(es)
negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation regulation of transcription, DNA-templated mitochondrion organization signal transduction negative regulation of cell proliferation negative regulation of transcription by competitive promoter binding histone deacetylation negative regulation of cell growth negative regulation of protein catabolic process regulation of apoptotic process positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of complement activation protein stabilization progesterone receptor signaling pathway negative regulation of androgen receptor signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to interleukin-6 DNA biosynthetic process negative regulation of glucocorticoid receptor signaling pathway transcription from RNA polymerase II promoter negative regulation of apoptotic process DNA replication ovarian follicle development ovarian follicle atresia organ regeneration response to cytokine response to immobilization stress response to drug response to peptide hormone response to ethanol
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