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  • Primary Antibodies ›
  • Profilin 1 Antibodies

Invitrogen

Profilin 1 Monoclonal Antibody (OTI2D2)

Advanced Verification
1 Published Figure
2 References
View all (26) Profilin 1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Profilin 1 Monoclonal Antibody (OTI2D2)

  • Antibody Testing Data (11)
  • Published Figures (1)
  • Advanced Verification (3)
Profilin 1 Antibody in Immunocytochemistry (ICC/IF)
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Profilin 1 Antibody in Immunocytochemistry (ICC/IF)
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Profilin 1 Antibody (MA5-25120) in ICC/IF

Immunofluorescence analysis of Profilin 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Profilin 1 Monoclonal Antibody (OTI2D2) (Product # MA5-25120) at 1:100 dilution in 0.1% BSA, incubated at 4 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Profilin 1 Antibody in Immunocytochemistry (ICC/IF)
Profilin 1 Antibody in Immunocytochemistry (ICC/IF)
Profilin 1 Antibody in Immunocytochemistry (ICC/IF)
Profilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Profilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Profilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Profilin 1 Antibody in Western Blot (WB)
Profilin 1 Antibody in Western Blot (WB)
Profilin 1 Antibody in Western Blot (WB)
Profilin 1 Antibody in Western Blot (WB)
Profilin 1 Antibody in Western Blot (WB)
Profilin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)
Profilin 1 Antibody
Profilin 1 Antibody
Profilin 1 Antibody
Profilin 1 Monoclonal Antibody (OTI2D2)

Product Details

MA5-25120

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:100
-

Immunoprecipitation (IP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2D2

Immunogen

Full length human recombinant protein of PFN1 produced in HEK293T cell
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.00 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2725112

Target Information

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Epididymis tissue protein Li 184a; profilin 1; Profilin I; Profilin-1

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Gene Aliases: ALS18; PFN1

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UniProt ID: (Human) P07737, (Rat) P62963

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Entrez Gene ID: (Dog) 607397, (Human) 5216, (Rat) 64303

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Function(s)
adenyl-nucleotide exchange factor activity actin binding actin monomer binding receptor binding protein binding phosphatidylinositol-4,5-bisphosphate binding Rho GTPase binding poly(A) RNA binding proline-rich region binding cadherin binding involved in cell-cell adhesion
Process(es)
neural tube closure regulation of transcription from RNA polymerase II promoter actin polymerization or depolymerization response to organic substance positive regulation of epithelial cell migration negative regulation of actin filament polymerization positive regulation of actin filament polymerization negative regulation of actin filament bundle assembly positive regulation of ATPase activity positive regulation of transcription from RNA polymerase II promoter positive regulation of viral transcription positive regulation of DNA metabolic process positive regulation of stress fiber assembly negative regulation of stress fiber assembly cellular response to growth factor stimulus positive regulation of ruffle assembly actin cytoskeleton organization positive regulation of actin filament bundle assembly protein stabilization Wnt signaling pathway, planar cell polarity pathway cell-cell adhesion
It has to be done as per old AB suggested Products section.
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