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  • MUNC18 Antibodies

Invitrogen

Phospho-STXBP1 (Ser313) Polyclonal Antibody

View all (23) MUNC18 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-STXBP1 (Ser313) Polyclonal Antibody

  • Antibody Testing Data (2)
Phospho-STXBP1 (Ser313) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-STXBP1 (Ser313) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-STXBP1 (Ser313) Antibody (PA5-105191) in ICC/IF

Immunocytochemistry analysis of Phospho-STXBP1 (Ser313) in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-STXBP1 (Ser313) polyclonal antibody (Product # PA5-105191) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-STXBP1 (Ser313) Antibody in Immunocytochemistry (ICC/IF)
Phospho-STXBP1 (Ser313) Antibody in Western Blot (WB)

Product Details

PA5-105191

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human STXBP1(Accession P61764), corresponding to amino acid residues around phosphorylated Ser313.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816664

Product Specific Information

Antibody detects endogenous levels of MUNC-18a only when phosphorylated at Ser313.

Target Information

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EIEE4; FLJ37475; hUNC18; MGC146482; minisatellite 10g detected by probe MMS10; munc-18c; MUNC18-1; N-Sec1; neuronal SEC1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; rbSec1; rop; RP11-56D16.3; Syntaxin-binding protein 1; UNC-18 homolog; unc-18-1; Unc-18A; Unc18-1

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Gene Aliases: AI317162; AI326233; ANC18HA; MMS10-G; Ms10g; Munc-18a; MUNC18-1; N-sec1; NSEC1; NSEC1A; P67; Rb-sec1; RBSEC1; rbSec1A; rbSec1B; Sec1; STXBP1; Sxtbp1; UNC18; Unc18-1; UNC18A; Unc18h

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UniProt ID: (Human) P61764, (Mouse) O08599, (Rat) P61765

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Entrez Gene ID: (Human) 6812, (Mouse) 20910, (Rat) 25558

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Function(s)
SNARE binding protein binding syntaxin-1 binding protein kinase binding protein domain specific binding syntaxin binding identical protein binding poly(A) RNA binding protein N-terminus binding
Process(es)
platelet degranulation transport exocytosis vesicle docking involved in exocytosis neurotransmitter secretion neuromuscular synaptic transmission axon target recognition regulation of synaptic vesicle priming protein transport synaptic vesicle maturation vesicle-mediated transport synaptic vesicle fusion to presynaptic active zone membrane negative regulation of synaptic transmission, GABAergic negative regulation of neuron apoptotic process positive regulation of exocytosis positive regulation of calcium ion-dependent exocytosis protein stabilization long term synaptic depression platelet aggregation protein localization to plasma membrane glutamate secretion regulation of synaptic vesicle fusion to presynaptic membrane regulation of SNARE complex assembly
It has to be done as per old AB suggested Products section.
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