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  • SOX9 Antibodies

Invitrogen

Phospho-SOX9 (Ser181) Polyclonal Antibody

View all (54) SOX9 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Phospho-SOX9 (Ser181) Polyclonal Antibody

  • Antibody Testing Data (3)
Phospho-SOX9 (Ser181) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-SOX9 (Ser181) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-SOX9 (Ser181) Antibody (PA5-104914) in ICC/IF

Immunofluorescent analysis of Phospho-SOX9 (Ser181) in A549 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-SOX9 (Ser181) polyclonal antibody (Product # PA5-104914) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-SOX9 (Ser181) Antibody in Immunocytochemistry (ICC/IF)
Phospho-SOX9 (Ser181) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-SOX9 (Ser181) Antibody in Western Blot (WB)
Phospho-SOX9 (Ser181) Polyclonal Antibody

Product Details

PA5-104914

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SOX9(Accession P48436), corresponding to amino acid residues around phosphorylated Ser181.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816387

Product Specific Information

Antibody detects endogenous levels of SOX9 only when phosphorylated at Serine 181.

Target Information

SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mSox9; SOX 9; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY box 9; SRY-Box 9 ; SRY-box containing gene 9; SRY-related HMG-box, gene 9; Transcription factor SOX-9

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Gene Aliases: 2010306G03Rik; AV220920; CMD1; CMPD1; mKIAA4243; SOX-9; SOX9; SRA1; SRXX2; SRXY10

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UniProt ID: (Chicken) P48434, (Human) P48436, (Mouse) Q04887

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Entrez Gene ID: (Chicken) 374148, (Human) 6662, (Mouse) 20682

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Function(s)
DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein kinase activity protein binding beta-catenin binding protein kinase A catalytic subunit binding enhancer binding bHLH transcription factor binding protein heterodimerization activity pre-mRNA intronic binding transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
branching involved in ureteric bud morphogenesis heart valve development transcription, DNA-templated regulation of transcription, DNA-templated male gonad development cellular response to retinoic acid negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation cell fate specification epithelial to mesenchymal transition tissue homeostasis positive regulation of protein phosphorylation hair follicle development positive regulation of mesenchymal cell proliferation chondrocyte development negative regulation of immune system process heart valve morphogenesis heart valve formation endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy nucleosome assembly chromatin remodeling regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein complex assembly protein phosphorylation cytoskeleton organization signal transduction epidermal growth factor receptor signaling pathway Notch signaling pathway spermatogenesis heart development positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell cycle process positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration neural crest cell development positive regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion male germ-line sex determination cAMP-mediated signaling cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of ossification negative regulation of bone mineralization negative regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation otic vesicle formation endocrine pancreas development negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation lacrimal gland development protein localization to nucleus somatic stem cell population maintenance intrahepatic bile duct development regulation of cell proliferation negative regulation of apoptotic process protein kinase B signaling cell fate commitment regulation of cell differentiation negative regulation of myoblast differentiation positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of photoreceptor cell differentiation oligodendrocyte differentiation homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development astrocyte fate commitment retina development in camera-type eye retinal rod cell differentiation endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation prostate gland morphogenesis epithelial cell proliferation involved in prostatic bud elongation bronchus cartilage development trachea cartilage development intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development regulation of branching involved in lung morphogenesis morphogenesis of a branching epithelium lung smooth muscle development negative regulation of biomineral tissue development ERK1 and ERK2 cascade Harderian gland development cellular response to mechanical stimulus cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus renal vesicle induction ureter development ureter smooth muscle cell differentiation ureter morphogenesis metanephric nephron tubule formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis positive regulation of extracellular matrix assembly positive regulation of male gonad development positive regulation of cell proliferation involved in heart morphogenesis positive regulation of mesenchymal stem cell differentiation regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process ossification prostate gland development mammary gland development notochord development regulation of apoptotic process limb bud formation cellular response to interleukin-1 ureter urothelium development
It has to be done as per old AB suggested Products section.
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