Invitrogen

Phospho-SMAD4 (Thr277) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Phospho-SMAD4 (Thr277) Polyclonal Antibody

Product Details

PA5-106038

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (IHC)

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD4(Accession Q13485), corresponding to amino acid residues around phosphorylated Thr277.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2817437

Product Specific Information

Antibody detects endogenous levels of Smad4 only when phosphorylated at Thr277, which site historically referenced as Thr276.

Target Information

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; hSMAD4; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4

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Gene Aliases: AW743858; D18Wsu70e; DPC4; JIP; MADH4; MYHRS; SMAD4

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UniProt ID: (Human) Q13485, (Mouse) P97471, (Rat) O70437

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Entrez Gene ID: (Human) 4089, (Mouse) 17128, (Rat) 50554

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding collagen binding transforming growth factor beta receptor, common-partner cytoplasmic mediator activity identical protein binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding protein heterodimerization activity I-SMAD binding R-SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding filamin binding SMAD binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovarian follicle development branching involved in ureteric bud morphogenesis response to hypoxia in utero embryonic development gastrulation with mouth forming second atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation left ventricular cardiac muscle tissue morphogenesis positive regulation of cell proliferation involved in heart valve morphogenesis brainstem development transcription from RNA polymerase II promoter cellular iron ion homeostasis transforming growth factor beta receptor signaling pathway SMAD protein complex assembly spermatogenesis single fertilization axon guidance endoderm development mesoderm development cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle hypertrophy positive regulation of epithelial to mesenchymal transition positive regulation of pathway-restricted SMAD protein phosphorylation neural crest cell differentiation regulation of transforming growth factor beta receptor signaling pathway negative regulation of cell growth BMP signaling pathway positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway somite rostral/caudal axis specification regulation of transforming growth factor beta2 production positive regulation of luteinizing hormone secretion somatic stem cell population maintenance intracellular signal transduction atrioventricular canal development endothelial cell activation embryonic digit morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of follicle-stimulating hormone secretion developmental growth neuron fate commitment sebaceous gland development formation of anatomical boundary regulation of binding positive regulation of histone H3-K4 methylation regulation of hair follicle development palate development uterus development positive regulation of SMAD protein import into nucleus SMAD protein signal transduction ventricular septum morphogenesis negative regulation of cell death endocardial cell differentiation female gonad morphogenesis interleukin-6-mediated signaling pathway negative regulation of ERK1 and ERK2 cascade response to transforming growth factor beta cellular response to BMP stimulus metanephric mesenchyme morphogenesis nephrogenic mesenchyme morphogenesis seminiferous tubule development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus positive regulation of histone H3-K9 acetylation kidney development cardiac septum development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter gastrulation anterior/posterior pattern specification regulation of cell proliferation negative regulation of protein catabolic process tissue morphogenesis
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