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AbboMax

Phospho-RhoGDI (Tyr156) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Phospho-RhoGDI (Tyr156) Polyclonal Antibody

  • Antibody Testing Data (1)
Phospho-RhoGDI (Tyr156) Antibody in Western Blot (WB)
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Phospho-RhoGDI (Tyr156) Antibody in Western Blot (WB)
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Phospho-RhoGDI (Tyr156) Antibody (620-550) in WB

Western Blot: The whole cell lysate derived from serum depleted MCF7 (Lane 1) and HELA (Lane 2) were separated in 12% SDS-PAGE and transferred onto NC membrane followed by immuoblotting of Rabbit anti-RhoGDI(pTyr156) (Cat#620-550) at 1:500. An immunoreactive band at ~24 kDa was observed. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-RhoGDI (Tyr156) Antibody in Western Blot (WB)
Phospho-RhoGDI (Tyr156) Polyclonal Antibody

Product Details

620-550

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide surrounding the epitope - CRPEEYEFL- with a phosphorylation site at Tyrosine 156 of human Rho-GDI alpha protein. The antigen corresponds to amino acid range 151-159 of the target protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

25mM tris with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive control: MCF7

Cellular location: Cytoplasmic.

Target Information

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: arhgdia {ECO:0000312|RGD:1359547}; epididymis secretory sperm binding protein Li 47e; GDI-1; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; rho GDI 1 {ECO:0000250|UniProtKB:P19803}; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP-dissociation inhibitor 1; rho GDP-dissociation inhibitor 1 {ECO:0000250|UniProtKB:P19803}; Rho-GDI alpha; rho-GDI alpha {ECO:0000250|UniProtKB:P19803}

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Gene Aliases: 5330430M07Rik; ARHGDIA; Bles01; C87222; Gdi-1; Gdi1; GDIA1; HEL-S-47e; NPHS8; Rho-GDI; RhoDGI; RHOGDI; RHOGDI-1

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UniProt ID: (Human) P52565, (Rat) Q5XI73, (Mouse) Q99PT1

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Entrez Gene ID: (Human) 396, (Rat) 360678, (Mouse) 192662

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Function(s)
Rho GDP-dissociation inhibitor activity GTPase activator activity protein binding fatty acid binding Rho GTPase binding protein kinase binding Hsp90 protein binding
Process(es)
Rho protein signal transduction regulation of cell shape negative regulation of cell migration regulation of protein localization positive regulation of GTPase activity regulation of catalytic activity cellular response to mechanical stimulus cellular response to organic cyclic compound cellular response to redox state semaphorin-plexin signaling pathway movement of cell or subcellular component negative regulation of cell adhesion regulation of Rho protein signal transduction negative regulation of apoptotic process negative regulation of axonogenesis positive regulation of axonogenesis regulation of small GTPase mediated signal transduction regulation of actin cytoskeleton reorganization
It has to be done as per old AB suggested Products section.
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