Invitrogen

Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Polyclonal Antibody

Product Details

PA5-114638

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RPS6KA1(Accession Q15418), corresponding to amino acid residues around phosphorylated Ser221.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2899274

Product Specific Information

Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.

Target Information

The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase domains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; 90 kDa ribosomal protein S6 kinase 4; 90 kDa ribosomal protein S6 kinase 6; coiled-coil domain containing 88B; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1C; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK-1c; MAPKAPK1A; MAPKAPK1D; mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase-2; mitogen-activated protein kinase-activated protein kinase 1C; Nuclear mitogen- and stress-activated protein kinase 2; p90 rsk; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK 6; p90RSK1; p90RSK2; p90RSK6; p90S6K; pp90RSK1; pp90RSK2; pp90RSK3; pp90RSK4; Protein-tyrosine kinase Mpk-9; Ribosomal protein kinase B; ribosomal protein S6 kinase 2; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 2; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-6; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase, 90kD, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 6; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; Ribosomal S6 kinase 4; RLSK; RP11-393H10.3; RSK-1; RSK-2; RSK-3; RSK-4; RSK-like protein kinase; RSKB; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha 2; S6K-alpha 6; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha-4; S6K-alpha-6

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Gene Aliases: 1110069D02Rik; 2610524K04Rik; 90kDa; AI848992; C130006E23; Ccdc88b; CLS; D17Wsu134e; HU-1; HU-2; HU-3; ISPK-1; ISPK1; Mapkapk-1a; MAPKAPK-1b; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; mMSK2; MPK-9; MRX19; MSK2; p90-Rsk1; p90-RSK2; p90-RSK3; p90Rsk; p90Rsk1; p90RSK2; p90RSK3; p90S6K; pp90rsk; pp90RSK2; pp90RSK3; PP90RSK4; RGD1560817; RGD1563860; Rps6ka-rs1; RPS6KA1; RPS6KA2; RPS6KA3; RPS6KA4; RPS6KA6; RSK; Rsk-1; RSK-B; RSK1; RSK2; RSK3; RSK4; S6K-alpha; S6K-alpha-1; S6K-alpha-4; S6K-alpha-6; S6K-alpha2; S6K-alpha3

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UniProt ID: (Human) Q15418, (Human) Q15349, (Human) P51812, (Human) O75676, (Human) Q9UK32, (Rat) Q63531, (Mouse) P18653, (Mouse) Q9WUT3, (Mouse) P18654, (Mouse) Q9Z2B9, (Mouse) Q7TPS0

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Entrez Gene ID: (Human) 6195, (Human) 6196, (Human) 6197, (Human) 8986, (Human) 27330, (Rat) 81771, (Mouse) 20111, (Mouse) 20112, (Rat) 117269, (Rat) 501560, (Mouse) 110651, (Mouse) 56613, (Rat) 361715, (Mouse) 67071, (Rat) 317203

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Function(s)
magnesium ion binding protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process ribosomal protein S6 kinase activity protein kinase activity protein kinase binding histone kinase activity (H3-S10 specific) mitogen-activated protein kinase p38 binding transcription factor binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups molecular_function metal ion binding
Process(es)
regulation of translation protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator phosphorylation intracellular signal transduction negative regulation of embryonic development negative regulation of ERK1 and ERK2 cascade negative regulation of mesoderm development skeletal system development toll-like receptor signaling pathway apoptotic process cell cycle signal transduction central nervous system development positive regulation of cell growth response to lipopolysaccharide negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of translation in response to stress regulation of DNA-templated transcription in response to stress positive regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter oocyte maturation brain renin-angiotensin system heart development negative regulation of cell proliferation positive regulation of gene expression cardiac muscle cell apoptotic process peptidyl-serine phosphorylation positive regulation of apoptotic process negative regulation of cell cycle negative regulation of meiotic nuclear division heart contraction regulation of protein processing cellular response to carbohydrate stimulus regulation of transcription, DNA-templated inflammatory response positive regulation of histone phosphorylation positive regulation of histone acetylation histone H3-S10 phosphorylation histone H3-S28 phosphorylation interleukin-1-mediated signaling pathway biological_process hepatocyte proliferation positive regulation of hepatic stellate cell activation positive regulation of transcription, DNA-templated negative regulation of cytokine production histone phosphorylation positive regulation of CREB transcription factor activity positive regulation of NF-kappaB transcription factor activity
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