Invitrogen

Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody

View all (13) PI3K p85/p55 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody

Product Details

PA5-118549

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PIK3R1(Accession P27986), corresponding to amino acid residues around phosphorylated Tyr199.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2903051

Product Specific Information

Antibody detects endogenous levels of PI3K p85/p55 only when phosphorylated at Tyr467(p85) or Tyr199(p55). The site Tyr467 was historically referenced as Tyr458.

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000009783; OTTHUMP00000009784; p55PIK; phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55); Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase p55 subunit; phosphatidylinositol 3-kinase regulartory subunit polypeptide 2; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulartory subunit, polypeptide 2; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase-associated p-85 alpha; Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit beta; PI3-kinase p85 subunit gamma; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit beta; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3-kinase subunit p85-beta; PI3K regulatory subunit alpha; PI3K regulatory subunit beta; PI3K regulatory subunit gamma; polypeptide 3; ptdIns-3-kinase p85-alpha; ptdIns-3-kinase p85-beta; ptdIns-3-kinase p85-gamma; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-alpha; ptdIns-3-kinase regulatory subunit p85-beta; RP4-533D7.2

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Gene Aliases: AA414954; AGM7; GRB1; IMD36; MPPH; MPPH1; p50alpha; p55; p55-GAMMA; p55alpha; p55PIK; p85; p85-ALPHA; p85-BETA; p85alpha; P85B; p85beta; PI3K; PI3KA; PIK3R1; PIK3R2; PIK3R3

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UniProt ID: (Human) P27986, (Human) O00459, (Human) Q92569, (Mouse) P26450, (Rat) Q63787, (Mouse) O08908, (Rat) Q63789, (Mouse) Q64143

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Entrez Gene ID: (Human) 5295, (Human) 5296, (Human) 8503, (Mouse) 18708, (Rat) 25513, (Mouse) 18709, (Rat) 29741, (Rat) 60664, (Mouse) 18710

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Function(s)
transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity GTPase activator activity receptor tyrosine kinase binding receptor binding platelet-derived growth factor receptor binding calmodulin binding protein C-terminus binding protein kinase binding protein domain specific binding estrogen receptor binding ubiquitin protein ligase binding ATPase binding phosphoprotein binding kinase activity
Process(es)
insulin receptor signaling pathway phosphorylation regulation of phosphatidylinositol 3-kinase activity phosphatidylinositol biosynthetic process phosphatidylinositol-3-phosphate biosynthetic process positive regulation of glucose import in response to insulin stimulus cellular glucose homeostasis regulation of autophagy phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling protein transport cellular response to insulin stimulus response to endoplasmic reticulum stress Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of transcription factor import into nucleus positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol phosphorylation vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling T cell receptor signaling pathway leukocyte migration regulation of small GTPase mediated signal transduction positive regulation of protein phosphorylation negative regulation of cell-matrix adhesion glucose metabolic process protein phosphorylation transport negative regulation of cell adhesion signal transduction extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of heart rate positive regulation of gene expression negative regulation of muscle cell apoptotic process negative regulation of cell-cell adhesion B cell differentiation positive regulation of cell migration positive regulation of tumor necrosis factor production response to insulin positive regulation of RNA splicing cellular response to UV negative regulation of apoptotic process positive regulation of myoblast differentiation negative regulation of osteoclast differentiation negative regulation of blood pressure negative regulation of proteolysis insulin-like growth factor receptor signaling pathway negative regulation of smooth muscle cell proliferation protein stabilization response to glucocorticoid regulation of stress fiber assembly NFAT protein import into nucleus response to cAMP growth hormone receptor signaling pathway regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response epidermal growth factor receptor signaling pathway viral process platelet activation T cell costimulation ERBB2 signaling pathway positive regulation of glucose import positive regulation of establishment of protein localization to plasma membrane regulation of actin filament polymerization regulation of phosphorylation positive regulation of cell adhesion response to yeast aging response to nutrient response to fructose response to iron(II) ion response to estradiol response to progesterone response to testosterone response to drug response to amino acid response to ethanol response to fatty acid response to growth factor cellular response to fatty acid response to dexamethasone
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