Invitrogen

Phospho-MECP2 (Ser80) Polyclonal Antibody

View all (42) MECP2 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-143716

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100
-

Immunohistochemistry (Paraffin) (IHC (P))

1:250
-

Immunocytochemistry (ICC/IF)

1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

phospho-MeCP2 (Ser-80) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 80 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942944

Product Specific Information

The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686A24160; MeCp-2 protein; methyl CpG binding protein 2; Methyl-CpG-binding protein 2; testis tissue sperm-binding protein Li 41a

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Gene Aliases: 1500041B07Rik; AUTSX3; D630021H01Rik; Mbd5; MECP2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP10

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UniProt ID: (Human) P51608, (Mouse) Q9Z2D6, (Rat) Q00566

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Entrez Gene ID: (Human) 4204, (Mouse) 17257, (Rat) 29386

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Function(s)
four-way junction DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity mRNA binding protein binding transcription factor binding methyl-CpG binding double-stranded methylated DNA binding protein domain specific binding chromatin DNA binding siRNA binding histone deacetylase binding poly(A) RNA binding unmethylated CpG binding protein N-terminus binding chromatin binding enzyme binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter behavioral fear response response to hypoxia startle response neurological system process involved in regulation of systemic arterial blood pressure regulation of respiratory gaseous exchange by neurological system process inositol metabolic process mitochondrial electron transport, ubiquinol to cytochrome c chromatin silencing regulation of gene expression by genetic imprinting transcription, DNA-templated regulation of transcription, DNA-templated glutamine metabolic process cellular biogenic amine metabolic process chemical synaptic transmission synapse assembly brain development respiratory gaseous exchange learning memory long-term memory protein localization glucocorticoid metabolic process positive regulation of cell proliferation adult locomotory behavior visual learning pathogenesis embryo development post-embryonic development regulation of gene expression dendrite development histone methylation histone acetylation proprioception sensory perception of pain cerebellum development ventricular system development neuron differentiation negative regulation of histone methylation neuron projection development cardiolipin metabolic process multicellular organismal response to stress negative regulation of histone acetylation social behavior regulation of gene expression, epigenetic neuron maturation negative regulation of neuron apoptotic process regulation of DNA methylation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated phosphatidylcholine metabolic process regulation of synaptic plasticity negative regulation of astrocyte differentiation catecholamine secretion neuromuscular process controlling posture neuromuscular process positive regulation of synapse assembly excitatory postsynaptic potential long-term synaptic potentiation heart development response to ionizing radiation response to lead ion response to organic cyclic compound response to estradiol locomotory behavior response to radiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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