Invitrogen

Phospho-IRE1 alpha (Ser724) Recombinant Rabbit Monoclonal Antibody (PSH03-35)

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Datasheet
Protocols
Questions & Answers
Phospho-IRE1 alpha (Ser724) Recombinant Rabbit Monoclonal Antibody (PSH03-35)

Product Details

MA5-55600

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunocytochemistry (ICC/IF)

1:500
-

Flow Cytometry (Flow)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH03-35

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser724 of human ire1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive Control: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat cells treated with 100nM Calyculin A for 30 minutes, human pancreas tissue, HeLa cells treated with 100nM Calyculin A for 30 minutes.

Tissue Specificity: Tissue enhanced (adrenal gland, pancreas).

Subcellular Location: Endoplasmic reticulum membrane; nucleus.

Sequence Similarities: 100% Mouse.

Predicted band size: 110 kDa.

Target Information

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1

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Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P

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UniProt ID: (Human) Q59EE2, (Mouse) Q9EQY0

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Entrez Gene ID: (Human) 2081, (Mouse) 78943

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Function(s)
magnesium ion binding endoribonuclease activity protein serine/threonine kinase activity protein binding ATP binding enzyme binding Hsp70 protein binding identical protein binding protein homodimerization activity ADP binding Hsp90 protein binding nucleotide binding catalytic activity endonuclease activity ribonuclease activity protein kinase activity kinase activity bidentate ribonuclease III activity transferase activity transferase activity, transferring phosphorus-containing groups hydrolase activity metal ion binding endoribonuclease
Process(es)
endothelial cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated mRNA cleavage mRNA catabolic process protein phosphorylation activation of signaling protein activity involved in unfolded protein response cell cycle arrest activation of JUN kinase activity regulation of macroautophagy positive regulation of RNA splicing response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus peptidyl-serine autophosphorylation IRE1-mediated unfolded protein response protein autophosphorylation mRNA splicing, via endonucleolytic cleavage and ligation mRNA endonucleolytic cleavage involved in unfolded protein response intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to glucose stimulus RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of endoplasmic reticulum unfolded protein response insulin metabolic process peptidyl-serine trans-autophosphorylation mRNA processing apoptotic process response to unfolded protein metabolic process phosphorylation endoplasmic reticulum unfolded protein response mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response
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