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  • Histone H3 Antibodies

Invitrogen

Phospho-Histone H3 (Thr32) Polyclonal Antibody

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View all (191) Histone H3 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Phospho-Histone H3 (Thr32) Polyclonal Antibody

  • Antibody Testing Data (2)
  • Advanced Verification (2)
Phospho-Histone H3 (Thr32) Antibody in Western Blot (WB)
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Phospho-Histone H3 (Thr32) Antibody in Western Blot (WB)
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Phospho-Histone H3 (Thr32) Antibody (PA5-40141) in WB

Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), HeLa treated with Calyculin A (50 ng/mL for 30 minutes) (Lane 2), RAW 264.7 (Lane 3), RAW 264.7 treated with Calyculin A (50 ng/mL for 30 minutes) (Lane 4), NIH/3T3 (Lane 5) and NIH/3T3 treated with Calyculin A (50 ng/mL for 30 minutes) (Lane 6). The blot was probed with Anti-Phospho-Histone H3 (Thr32) (Product # PA5-40141... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-Histone H3 (Thr32) Antibody in Western Blot (WB)
Phospho-Histone H3 (Thr32) Antibody in ChIP Assay (ChIP)
Phospho-Histone H3 (Thr32) Antibody
Phospho-Histone H3 (Thr32) Antibody
Phospho-Histone H3 (Thr32) Polyclonal Antibody

Product Details

PA5-40141

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

ChIP assay (ChIP)

1.5 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of threonine 32(L-A-T(p)-K-A) derived from Human Histone H3.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608327

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3S10ph; histone 1, H2ai; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H2ai; histone cluster 1, H2ai-like; histone cluster 1, H3a; Histone Cluster 2 H3a; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone cluster 3, H3; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o

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Gene Aliases: H3; H3 histone; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; Hist1h2ai; Hist1h2ail; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Rat) Q6LED0, (Mouse) P68433, (Mouse) P84228

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Rat) 291159, (Mouse) 360198, (Rat) 679994, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114, (Rat) 310678

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Function(s)
DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion chromatin binding
Process(es)
positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing chromatin silencing at rDNA blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic telomere organization cell-cell adhesion xenophagy negative regulation of transcription from RNA polymerase II promoter
It has to be done as per old AB suggested Products section.
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