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  • GSK3B Antibodies

Invitrogen

Phospho-GSK3B (Thr390) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-GSK3B (Thr390) Polyclonal Antibody

  • Antibody Testing Data (12)
Phospho-GSK3B (Thr390) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-GSK3B (Thr390) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-GSK3B (Thr390) Antibody (PA5-114578) in ICC/IF

Immunocytochemistry analysis of Phospho-GSK3B (Thr390) in A549 cells (4h of LPS treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114578). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjug... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-GSK3B (Thr390) Antibody in Immunocytochemistry (ICC/IF)
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-GSK3B (Thr390) Antibody in Western Blot (WB)
Phospho-GSK3B (Thr390) Polyclonal Antibody

Product Details

PA5-114578

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human GSK3 beta around the phosphorylation site of Thr390.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2899214

Product Specific Information

Antibody detects endogenous levels of GSK3 beta only when phosphorylated at Thr390.

Target Information

GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3beta isoform; pp37; Serine/threonine-protein kinase GSK3B

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Gene Aliases: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3B

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UniProt ID: (Human) P49841, (Mouse) Q9WV60, (Rat) P18266

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Entrez Gene ID: (Human) 2932, (Mouse) 56637, (Rat) 84027

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Function(s)
RNA polymerase II transcription factor binding p53 binding protein kinase activity protein serine/threonine kinase activity integrin binding protein binding ATP binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding ionotropic glutamate receptor binding tau protein binding tau-protein kinase activity NF-kappaB binding nucleotide binding transcription factor binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of signal transduction negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation cell migration peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation protein localization to microtubule intracellular signal transduction cellular response to interleukin-3 positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of stem cell differentiation dopamine receptor signaling pathway aging positive regulation of mitochondrial membrane potential response to activity peptidyl-threonine phosphorylation response to estradiol response to insulin negative regulation of smooth muscle cell apoptotic process cellular response to hepatocyte growth factor stimulus cellular response to erythropoietin response to drug proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of smooth muscle cell proliferation negative regulation of nitric-oxide synthase activity intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to iron(II) ion cellular response to lithium ion response to epinephrine negative regulation of canonical Wnt signaling pathway chemical synaptic transmission, postsynaptic regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin negative regulation of type B pancreatic cell development positive regulation of excitatory postsynaptic potential negative regulation of glycogen (starch) synthase activity positive regulation of DNA biosynthetic process negative regulation of neuron migration response to lithium ion
It has to be done as per old AB suggested Products section.
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