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  • GSK3 alpha/beta Antibodies

Invitrogen

Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Rabbit Monoclonal Antibody (19H1L12)

Advanced Verification
2 Published Figures
1 Reference
View all (17) GSK3 alpha/beta antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Rabbit Monoclonal Antibody (19H1L12)

  • Antibody Testing Data (2)
  • Published Figures (2)
  • Advanced Verification (1)
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 5

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Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody (702230) in ICC/IF

For immunofluorescence analysis, 3T3-L1 cells were fixed and permeabilized for detection of endogenous CSL/RBP J using GSK-3 alpha (pY279) + GSK3 beta (pY216) Recombinant Rabbit Monoclonal Antibody (Product # 702230, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunocytochemistry (ICC/IF)
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Western Blot (WB)
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunohistochemistry (IHC)
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunohistochemistry (IHC)
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Rabbit Monoclonal Antibody (19H1L12)

Product Details

702230

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

5 µg x 10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

19H1L12

Immunogen

Peptide corresponding to Human GSK3A, GSK3B (aa 211-220)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2632971

Product Specific Information

This antibody is predicted to react with Monkey and galago.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3beta isoform; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

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Gene Aliases: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3A; GSK3B

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UniProt ID: (Human) P49840, (Human) P49841, (Mouse) Q2NL51, (Mouse) Q9WV60

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Entrez Gene ID: (Human) 2931, (Human) 2932, (Mouse) 606496, (Mouse) 56637

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Function(s)
protein serine/threonine kinase activity protein binding ATP binding protein kinase A catalytic subunit binding tau-protein kinase activity RNA polymerase II transcription factor binding p53 binding protein kinase activity integrin binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding ionotropic glutamate receptor binding tau protein binding NF-kappaB binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups transcription factor binding
Process(es)
re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of signal transduction negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation cell migration peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation protein localization to microtubule intracellular signal transduction cellular response to interleukin-3 positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of stem cell differentiation dopamine receptor signaling pathway aging positive regulation of mitochondrial membrane potential response to activity peptidyl-threonine phosphorylation response to estradiol response to insulin negative regulation of smooth muscle cell apoptotic process cellular response to hepatocyte growth factor stimulus cellular response to erythropoietin response to drug proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of smooth muscle cell proliferation negative regulation of nitric-oxide synthase activity intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to iron(II) ion cellular response to lithium ion response to epinephrine negative regulation of canonical Wnt signaling pathway chemical synaptic transmission, postsynaptic regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin negative regulation of type B pancreatic cell development positive regulation of excitatory postsynaptic potential negative regulation of glycogen (starch) synthase activity positive regulation of DNA biosynthetic process negative regulation of neuron migration regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis insulin receptor signaling pathway negative regulation of UDP-glucose catabolic process positive regulation of cAMP biosynthetic process negative regulation of TOR signaling cellular response to insulin stimulus IRE1-mediated unfolded protein response positive regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of cell growth involved in cardiac muscle cell development positive regulation of adrenergic receptor signaling pathway regulation of mitophagy positive regulation of protein targeting to mitochondrion negative regulation of glycogen synthase activity, transferring glucose-1-phosphate positive regulation of glycogen (starch) synthase activity negative regulation of transferase activity cellular response to organic cyclic compound
It has to be done as per old AB suggested Products section.
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