Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Primary Antibodies ›
  • GATA3 Antibodies

Invitrogen

Phospho-GATA3 (Ser308) Recombinant Rabbit Monoclonal Antibody (9L8O7)

View all (114) GATA3 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-GATA3 (Ser308) Recombinant Rabbit Monoclonal Antibody (9L8O7)

  • Antibody Testing Data (1)
Phospho-GATA3 (Ser308) Antibody in Western Blot (WB)
Group 53 Created with Sketch.
Phospho-GATA3 (Ser308) Antibody in Western Blot (WB)
Group 53 Created with Sketch.

FIGURE: 1 / 1

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

Phospho-GATA3 (Ser308) Antibody (MA5-35863) in WB

Western blot analysis of Phospho-GATA3 (Ser308) in extracts of RAW264.7 cells. Samples were incubated with Phospho-GATA3 (Ser308) Monoclonal antibody (Product # MA5-35863) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. RAW264.7 cells were treated by Doxorubicin (0.5 µM) at 37°C for 24 hours. Lysates/proteins: 25 µg per lane. Blocking buf... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

Phospho-GATA3 (Ser308) Antibody in Western Blot (WB)
Phospho-GATA3 (Ser308) Recombinant Rabbit Monoclonal Antibody (9L8O7)

Product Details

MA5-35863

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9L8O7

Immunogen

A phospho specific peptide corresponding to residues surrounding S308 of human GATA3
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849761

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

View more View less

Gene Aliases: Gata-3; Gata3; jal

View more View less

UniProt ID: (Mouse) P23772

View more View less

Entrez Gene ID: (Mouse) 14462

View more View less

Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding protein dimerization activity E-box binding HMG box domain binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cell morphogenesis in utero embryonic development cell fate determination neuron migration cell activation positive regulation of cytokine production kidney development mesonephros development lens development in camera-type eye immune system process pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter humoral immune response signal transduction nervous system development axon guidance negative regulation of cell proliferation male gonad development post-embryonic development positive regulation of signal transduction positive regulation of endothelial cell migration negative regulation of gene expression regulation of neuron projection development phosphatidylinositol 3-kinase signaling neuron differentiation T cell differentiation erythrocyte differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production T cell differentiation in thymus negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process norepinephrine biosynthetic process inner ear morphogenesis regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process ear development thymic T cell selection T-helper 2 cell differentiation innate immune response positive regulation of T cell differentiation positive regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development embryonic organ development developmental growth anatomical structure formation involved in morphogenesis homeostasis of number of cells negative regulation of inflammatory response T cell receptor signaling pathway regulation of histone H3-K4 methylation positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development mesenchymal to epithelial transition mast cell differentiation ureteric bud formation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to cytokine stimulus cellular response to interleukin-4 otic vesicle development cellular response to BMP stimulus renal system development positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation interleukin-4 secretion interferon-gamma secretion lymphocyte migration regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-55c564d944-rwwh4:80/100.66.128.142:80.
git-commit: 3092084cb0e494f0e07f07633350183de7fa10a8
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0