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  • EEF2 Antibodies

Invitrogen

Phospho-EEF2 (Thr57) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Phospho-EEF2 (Thr57) Polyclonal Antibody

  • Antibody Testing Data (5)
Phospho-EEF2 (Thr57) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Phospho-EEF2 (Thr57) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Phospho-EEF2 (Thr57) Antibody (PA5-105491) in IHC (P)

Immunohistochemistry analysis of paraffin-embedded Phospho-EEF2 (Thr57) in mouse spleen tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-EEF2 (Thr57) polyclonal antibody (Product # PA5-105491) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-EEF2 (Thr57) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EEF2 (Thr57) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EEF2 (Thr57) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EEF2 (Thr57) Antibody in Western Blot (WB)
Phospho-EEF2 (Thr57) Antibody in Western Blot (WB)
Phospho-EEF2 (Thr57) Polyclonal Antibody

Product Details

PA5-105491

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human EEF2(Accession P13639), corresponding to amino acid residues around phosphorylated Thr57.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816919

Product Specific Information

Antibody detects endogenous levels of EEF2 only when phosphorylated at Thr57.

Target Information

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EF-2; Elongation factor 2; elongation factor-2; polypeptidyl-tRNA translocase

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Gene Aliases: EEF-2; EEF2; EF-2; EF2; SCA26

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UniProt ID: (Human) P13639, (Mouse) P58252, (Rat) P05197

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Entrez Gene ID: (Human) 1938, (Mouse) 13629, (Rat) 29565

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Function(s)
p53 binding translation elongation factor activity GTPase activity protein binding GTP binding 5S rRNA binding protein kinase binding ribosome binding poly(A) RNA binding actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding translation activator activity
Process(es)
hematopoietic progenitor cell differentiation translation translational elongation positive regulation of translation response to ischemia skeletal muscle contraction aging glial cell proliferation response to fluoxetine peptidyl-diphthamide biosynthetic process from peptidyl-histidine response to estradiol response to endoplasmic reticulum stress skeletal muscle cell differentiation response to hydrogen peroxide response to ethanol response to folic acid cell-cell adhesion cellular response to brain-derived neurotrophic factor stimulus positive regulation of cytoplasmic translation
It has to be done as per old AB suggested Products section.
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