Invitrogen

Phospho-Chk2 (Thr26, Ser28) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Phospho-Chk2 (Thr26, Ser28) Polyclonal Antibody

Product Details

PA5-105774

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CHEK2(Accession O96017), corresponding to amino acid residues around phosphorylated Ser28.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2817173

Product Specific Information

Antibody detects endogenous levels of Chk2 only when phosphorylated at Thr26+Ser28.

Target Information

CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; checkpoint-like protein CHK2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; del9; EC 2.7.11.1; HuCds 1; Hucds1; kinase Chk2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; protein kinase Chk2; Rad53 homolog; RP11-436C9.1; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2

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Gene Aliases: CDS1; CHEK2; CHK2; hCds1; HUCDS1; LFS2; PP1425; RAD53

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UniProt ID: (Human) Q6QA04, (Mouse) Q9Z265

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Entrez Gene ID: (Human) 11200, (Mouse) 50883, (Rat) 114212

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Function(s)
protein serine/threonine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity metal ion binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
DNA damage checkpoint G2/M transition of mitotic cell cycle replicative cell aging DNA repair double-strand break repair transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation apoptotic process cellular response to DNA damage stimulus DNA damage induced protein phosphorylation cell cycle mitotic nuclear division intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation phosphorylation regulation of protein catabolic process signal transduction in response to DNA damage cellular protein catabolic process positive regulation of transcription, DNA-templated protein autophosphorylation protein stabilization cell division signal transduction involved in intra-S DNA damage checkpoint mitotic spindle assembly positive regulation of protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest peptidyl-serine phosphorylation cellular response to drug negative regulation of cell cycle arrest replicative senescence regulation of signal transduction by p53 class mediator response to doxorubicin cellular response to bisphenol A negative regulation of DNA damage checkpoint positive regulation of anoikis
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