Invitrogen

Phospho-Chk1 (Ser296) Polyclonal Antibody

Name: Phospho-Chk1 (Ser296) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-104213

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)

Phospho-Chk1 (Ser296) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-Chk1 (Ser296) Antibody (PA5-104213) in IHC (P)

Immunohistochemistry analysis of paraffin-embedded Phospho-Chk1 (Ser296) in human Cholangio-carcinoma tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Chk1 (Ser296) polyclonal antibody (Product # PA5-104213) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Chk1 (Ser296) Antibody in Western Blot (WB)

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Immunogen Image Phospho-Chk1 (Ser296) Polyclonal Antibody

Product Details

PA5-104213

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CHEK1(Accession O14757), corresponding to amino acid residues around phosphorylated Ser296.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2815998

Product Specific Information

Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 296.

Target Information

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1; Synoviolin

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Gene Aliases: C85740; CHEK1; CHK1; rad27

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UniProt ID: (Human) O14757, (Rat) Q91ZN7, (Mouse) O35280

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Entrez Gene ID: (Human) 1111, (Rat) 140583, (Mouse) 12649

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity histone kinase activity (H3-T11 specific) nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

DNA damage checkpoint G2/M transition of mitotic cell cycle DNA repair protein phosphorylation cellular response to DNA damage stimulus DNA damage induced protein phosphorylation regulation of gene expression regulation of double-strand break repair via homologous recombination regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage negative regulation of G2/M transition of mitotic cell cycle peptidyl-threonine phosphorylation histone H3-T11 phosphorylation regulation of cell proliferation negative regulation of mitotic nuclear division regulation of mitotic centrosome separation chromatin-mediated maintenance of transcription cellular response to mechanical stimulus cellular response to organic substance cellular response to caffeine negative regulation of DNA biosynthetic process regulation of histone H3-K9 acetylation cell cycle phosphorylation G2 DNA damage checkpoint DNA replication replicative senescence regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-Chk1 (Ser296) Polyclonal Antibody

Phospho-Chk1 (Ser296) Antibody (PA5-104213) in IHC (P)

Product Details

PA5-104213

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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