Invitrogen

Phospho-Aurora A (Thr288) Polyclonal Antibody

Name: Phospho-Aurora A (Thr288) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-97372

View all (41) Aurora A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Phospho-Aurora A (Thr288) Antibody in Western Blot (WB)

FIGURE: 1 / 1

Phospho-Aurora A (Thr288) Antibody (PA5-97372) in WB

Western blot analysis of Phospho-Aurora A (Thr288) in extracts of HeLa and HT-29 cells. Samples were incubated with Phospho-Aurora A (Thr288) Polyclonal antibody (Product # PA5-97372) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by Hydroxyurea (4 mM) for 20 hours. HeLa cells were treated by Nocodazole (50 ng/mL)... View More

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Immunogen image

Immunogen Image Phospho-Aurora A (Thr288) Polyclonal Antibody

Product Details

PA5-97372

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T288 of human AURKA (NP_0035912)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.81 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2809174

Product Specific Information

Positive Samples: HeLa, HT-29; Cellular Location: Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole

Immunogen sequence: RTTLC

Target Information

AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ARK-1; AURAK; Aurora 2; Aurora A Kinase; Aurora kinase A; aurora/IP; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; EC 2.7.11.1; hARK1; Ipl1- and aurora-related kinase 1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; RP5-1167H4.6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1

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Gene Aliases: AIK; AIRK1; ARK1; AURA; AURKA; AYK1; BTAK; IAK1; PPP1R47; STK15; STK6; STK7

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UniProt ID: (Human) O14965

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Entrez Gene ID: (Human) 6790

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding histone serine kinase activity

Process(es)

G2/M transition of mitotic cell cycle meiotic spindle organization protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest spindle organization mitotic spindle organization spindle assembly involved in female meiosis I mitotic nuclear division mitotic centrosome separation anterior/posterior axis specification anaphase-promoting complex-dependent catabolic process regulation of protein stability negative regulation of protein binding response to estradiol positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of cytokinesis histone-serine phosphorylation protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process positive regulation of mitotic nuclear division regulation of centrosome cycle protein autophosphorylation cell division centrosome localization protein localization to centrosome positive regulation of oocyte maturation regulation of signal transduction by p53 class mediator neuron projection extension

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Phospho-Aurora A (Thr288) Polyclonal Antibody

Phospho-Aurora A (Thr288) Antibody (PA5-97372) in WB

Product Details

PA5-97372

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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