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  • AKT1 Antibodies

Invitrogen

Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal Antibody (99H9L9)

Advanced Verification
2 Published Figures
2 References
View all (186) AKT1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal Antibody (99H9L9)

  • Antibody Testing Data (2)
  • Published Figures (2)
  • Advanced Verification (1)
Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-AKT1 (Ser473) Antibody (700256) in ICC/IF

Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with AKT (pS473) Recombinant Rabbit Monoclonal Antibody (Product # 700256) at 1 µg-2 µg in 1% BSA and incubated ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
Phospho-AKT1 (Ser473) Antibody

Product Details

700256

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg
View 1 publication 1 publication

ELISA (ELISA)

1-5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

99H9L9

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532308

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

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Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

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UniProt ID: (Human) P31749, (Mouse) P31750

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Entrez Gene ID: (Human) 207, (Mouse) 11651

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding
Process(es)
protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation carbohydrate metabolic process glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation protein phosphorylation negative regulation of protein kinase activity transport apoptotic process activation-induced cell death of T cells inflammatory response cytoskeleton organization signal transduction multicellular organism development germ cell development nervous system development aging insulin receptor signaling pathway apoptotic mitochondrial changes carbohydrate transport response to hormone negative regulation of autophagy negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide cellular protein modification process nitric oxide biosynthetic process cellular response to DNA damage stimulus response to oxidative stress G-protein coupled receptor signaling pathway cell proliferation response to heat negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling peptidyl-threonine phosphorylation spinal cord development cell differentiation platelet activation hyaluronan metabolic process endocrine pancreas development T cell costimulation positive regulation of endodeoxyribonuclease activity ERBB2 signaling pathway regulation of mRNA stability positive regulation of epidermal growth factor receptor signaling pathway protein autophosphorylation phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity response to growth hormone mammary gland epithelial cell differentiation establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
It has to be done as per old AB suggested Products section.
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