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  • Pannexin 1 Antibodies

Alomone Labs, LTD

Pannexin 1 Polyclonal Antibody

View all (14) Pannexin 1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Pannexin 1 Polyclonal Antibody

  • Antibody Testing Data (2)
Pannexin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Pannexin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Pannexin 1 Antibody (ACC-234-200UL) in IHC (F)

Expression of Pannexin 1 in rat cortex - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Pannexin 1 Antibody (#ACC-234), (1:300). PANX1 staining (green) is apparent in cells with neuronal outline (vertical arrows) and apical dendrites (horizontal arrows) in the parietal cingulated cortex region. Cell nuclei are stained with DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Pannexin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))
Pannexin 1 Antibody in Western Blot (WB)
Pannexin 1 Polyclonal Antibody

Product Details

ACC-234-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:300
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1, Intracellular, N-terminus
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

The pannexin gene family encodes a second class of putative gap junction proteins and are highly conserved in invertebrates and mammals. Pannexins (Panx) are four-pass transmembrane proteins that oligomerize to form large pore ion and metabolite-permeable channels. Pannexin-1 (PANX1) and Pannexin-3 are closely related, while Pannexin-2 is a more distant relation. PANX1 is a transmembrane protein that forms a mechanosensitive ATP-permeable channel between adjacent cells and in the endoplasmic reticulum. PANX1 may play a role as a Ca2+ -leak channel to regulate ER Ca2+ homeostasis and regulates neural stem and progenitor cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: innexin; Pannexin-1; PANX1

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Gene Aliases: AI847747; MRS1; PANX1; PX1; UNQ2529; UNQ2529/PRO6028

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UniProt ID: (Human) Q543A0, (Rat) P60570, (Mouse) Q9JIP4

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Entrez Gene ID: (Human) 24145, (Rat) 315435, (Mouse) 55991

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Function(s)
protease binding receptor binding calcium channel activity leak channel activity ion channel binding protein heterodimerization activity actin filament binding gap junction hemi-channel activity scaffold protein binding actin binding gap junction channel activity protein binding channel activity wide pore channel activity
Process(es)
response to ischemia cation transport calcium ion transport cell-cell signaling response to ATP protein hexamerization positive regulation of cytokine secretion positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion transmembrane transport calcium ion transmembrane transport transport ion transport
It has to be done as per old AB suggested Products section.
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