OriGene

PTK2 Monoclonal Antibody (OTI4G7), TrueMAB™

View all (101) FAK antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
PTK2 Monoclonal Antibody (OTI4G7), TrueMAB™

Product Details

TA506182

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4G7

Immunogen

Full length human recombinant protein of human PTK2 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

View more View less

Gene Aliases: FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2

View more View less

UniProt ID: (Human) Q05397

View more View less

Entrez Gene ID: (Human) 5747

View more View less

Function(s)
actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding
Process(es)
MAPK cascade microtubule cytoskeleton organization angiogenesis vasculogenesis neuron migration placenta development regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis cellular component disassembly involved in execution phase of apoptosis signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway axon guidance positive regulation of cell proliferation regulation of cell shape embryo development regulation of endothelial cell migration regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion establishment of cell polarity extracellular matrix organization positive regulation of cell migration regulation of cell adhesion mediated by integrin netrin-activated signaling pathway peptidyl-tyrosine autophosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process regulation of GTPase activity endothelial cell migration positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of osteoblast differentiation positive regulation of protein kinase activity negative regulation of organ growth protein autophosphorylation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway cell motility negative regulation of axonogenesis regulation of cytoskeleton organization regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly growth hormone receptor signaling pathway regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support