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  • PPAR gamma Antibodies

Bethyl Laboratories

PPAR-gamma Polyclonal Antibody

View all (30) PPAR gamma antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite PPAR-gamma Polyclonal Antibody

  • Antibody Testing Data (3)
PPAR-gamma Antibody in Western Blot (WB)
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PPAR-gamma Antibody in Western Blot (WB)
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PPAR-gamma Antibody (A304-460A) in WB

Detection of mouse PPAR-gamma by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, differentiated mouse NIH 3T3-L1, and mouse NIH3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody (Product # A304-460A) (lot A304-460A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PPAR-gamma Antibody in Western Blot (WB)
PPAR-gamma Antibody in Western Blot (WB)
PPAR-gamma Antibody in Immunoprecipitation (IP)
PPAR-gamma Polyclonal Antibody

Product Details

A304-460A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:10,000-1:25,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 50 to 100 of Human Peroxisome Proliferator-Activated Receptor Gamma.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HUMPPARG; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARy

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Gene Aliases: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2

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UniProt ID: (Human) P37231, (Mouse) P37238

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Entrez Gene ID: (Human) 5468, (Mouse) 19016

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Function(s)
core promoter sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding retinoid X receptor binding arachidonic acid binding alpha-actinin binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding metal ion binding WW domain binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter placenta development negative regulation of acute inflammatory response transcription initiation from RNA polymerase II promoter lipid metabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction G-protein coupled receptor signaling pathway heart development response to nutrient regulation of blood pressure response to cold response to mechanical stimulus macrophage derived foam cell differentiation negative regulation of macrophage derived foam cell differentiation negative regulation of receptor biosynthetic process negative regulation of cholesterol storage negative regulation of sequestering of triglyceride long-chain fatty acid transport fatty acid oxidation monocyte differentiation negative regulation of cell growth epithelial cell differentiation response to caffeine organ regeneration response to retinoic acid cellular response to insulin stimulus negative regulation of collagen biosynthetic process response to vitamin A response to lipid peroxisome proliferator activated receptor signaling pathway response to immobilization stress response to diuretic glucose homeostasis response to starvation regulation of circadian rhythm lipoprotein transport steroid hormone mediated signaling pathway response to estrogen innate immune response cell fate commitment positive regulation of fat cell differentiation low-density lipoprotein particle receptor biosynthetic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation cell maturation rhythmic process negative regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation white fat cell differentiation positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of telomerase activity lipid homeostasis response to low-density lipoprotein particle positive regulation of phagocytosis, engulfment negative regulation of interferon-gamma-mediated signaling pathway regulation of cholesterol transporter activity regulation of transcription involved in cell fate commitment cellular response to retinoic acid cellular response to vitamin E cellular response to prostaglandin E stimulus cellular response to hyperoxia response to metformin negative regulation of pancreatic stellate cell proliferation negative regulation of cytokine production response to dietary excess diet induced thermogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response negative regulation of cell proliferation response to light stimulus regulation of gene expression regulation of lipid metabolic process intracellular receptor signaling pathway response to food positive regulation of apoptotic process fat cell differentiation regulation of fat cell differentiation brown fat cell differentiation cellular response to lithium ion cellular response to organic cyclic compound negative regulation of peptide hormone secretion negative regulation of cellular response to insulin stimulus
It has to be done as per old AB suggested Products section.
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