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  • Primary Antibodies ›
  • POLR2J Antibodies

Invitrogen

POLR2J Polyclonal Antibody

View all (12) POLR2J antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2J Polyclonal Antibody

  • Antibody Testing Data (3)
POLR2J Antibody in Immunocytochemistry (ICC/IF)
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POLR2J Antibody in Immunocytochemistry (ICC/IF)
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POLR2J Antibody (PA5-95952) in ICC/IF

Immunofluorescence analysis of POLR2J in U2OS cells. Samples were incubated with POLR2J Polyclonal antibody (Product # PA5-95952). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2J Antibody in Immunocytochemistry (ICC/IF)
POLR2J Antibody in Immunocytochemistry (ICC/IF)
POLR2J Antibody in Western Blot (WB)
POLR2J Polyclonal Antibody

Product Details

PA5-95952

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_0062251)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2807754

Product Specific Information

Immunogen sequence: MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL LEERFRVAIK DKQEGIE; Positive Samples: K-562, Mouse thymus; Cellular Location: Nucleus

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II subunit J; DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11; DNA-directed RNA polymerase II subunit RPB11-a; polymerase (RNA) II (DNA directed) polypeptide I; polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa; polymerase (RNA) II subunit J; RNA polymerase II 13.3 kDa subunit; RNA polymerase II 4 (14 kDa subunit); RNA polymerase II subunit B11; RNA polymerase II subunit B11-a; RNA polymerase II subunit RPB14; RPB14

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Gene Aliases: 14.5kDa; hRPB14; Polr2i; POLR2J; POLR2J1; RPB11; RPB11A; RPB11m; Rpo2-4

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UniProt ID: (Human) P52435

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Entrez Gene ID: (Human) 5439, (Mouse) 20022

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Function(s)
RNA polymerase II activity DNA binding DNA-directed RNA polymerase activity protein binding LRR domain binding protein dimerization activity
Process(es)
mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription transcription, DNA-templated
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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