Invitrogen

PLK1 Antibody Cocktail

36 Published Figures
51 References
View all (67) PLK1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
PLK1 Antibody Cocktail

Product Details

33-1700

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 36 publications 36 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

1-5 µg/mL
View 6 publications 6 publications

ELISA (ELISA)

0.1-1.0 µg/mL
-

Immunoprecipitation (IP)

5 µg
View 5 publications 5 publications

Miscellaneous PubMed (MISC)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa/IgG2b, kappa

Class

Cocktail

Type

Antibody

Clone

PL6/PL2

Immunogen

A His-tagged Plk fusion protein (C-term Plk1) containing amino acids 402-603 of the human Plk1 protein(
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533104

Product Specific Information

33-1700 contains a cocktail of monoclonal mouse PLK1 containing clone PL6, isotype IgG1, kappa and clone PY20, isotype IgG2b, kappa.

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; Plx1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

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Gene Aliases: PLK; PLK1; plx1; STPK13; XELAEV_18047692mg

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UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673, (Xenopus) P70032

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Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515, (Xenopus) 380481

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation cell cycle mitotic nuclear division mitotic spindle assembly checkpoint regulation of mitotic cell cycle positive regulation of peptidyl-threonine phosphorylation phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process polar body extrusion after meiotic divisions negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization cell division positive regulation of ubiquitin-protein transferase activity synaptonemal complex disassembly protein localization to chromatin regulation of mitotic spindle assembly positive regulation of ubiquitin protein ligase activity response to antibiotic sister chromatid cohesion mitotic nuclear envelope disassembly cell proliferation anaphase-promoting complex-dependent catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of cell cycle positive regulation of protein localization to nucleus regulation of cell cycle G2/M phase transition
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