OriGene

PLK1 Monoclonal Antibody (OTI1A2), TrueMAB™

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Datasheet
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Questions & Answers
Datasheet
Protocols
Questions & Answers
PLK1 Monoclonal Antibody (OTI1A2), TrueMAB™

Product Details

TA500395

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Flow Cytometry (Flow)

Assay-dependent
-

Immunoprecipitation (IP)

2 µg/500 µL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI1A2

Immunogen

Full length human recombinant protein of human PLK1 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.9 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

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Gene Aliases: PLK; PLK1; STPK13

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UniProt ID: (Human) P53350

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Entrez Gene ID: (Human) 5347

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding
Process(es)
mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation sister chromatid cohesion mitotic nuclear division mitotic nuclear envelope disassembly mitotic spindle assembly checkpoint regulation of mitotic cell cycle cell proliferation positive regulation of peptidyl-threonine phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition anaphase-promoting complex-dependent catabolic process G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein transferase activity regulation of cell cycle synaptonemal complex disassembly protein localization to chromatin positive regulation of protein localization to nucleus regulation of mitotic spindle assembly regulation of cell cycle G2/M phase transition positive regulation of ubiquitin protein ligase activity
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