Bethyl Laboratories

PDZ-GEF1 Polyclonal Antibody

View all (8) RAPGEF2 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
PDZ-GEF1 Polyclonal Antibody

Product Details

A301-966A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000 to 1:5,000
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 975 and 1025 of human PDZ domain containing guanine nucleotide exchange factor (GEF) 1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Dog

Target Information

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP- induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain- derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CNrasGEF; Cyclic nucleotide ras GEF; Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain containing guanine nucleotide exchange factor (GEF) 1; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA(Ras/Rap1A-associating)-GEF; RA-GEF-1; Rap guanine nucleotide exchange factor (GEF) 2; Rap guanine nucleotide exchange factor 2; Ras/Rap1-associating GEF-1

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Gene Aliases: CNrasGEF; KIAA0313; nRap GEP; NRAPGEP; PDZ-GEF1; PDZGEF1; RA-GEF; RA-GEF-1; Rap-GEP; RAPGEF2

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UniProt ID: (Human) Q9Y4G8

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Entrez Gene ID: (Human) 9693

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Function(s)
signal transducer activity Ras guanyl-nucleotide exchange factor activity GTPase activator activity calcium ion binding protein binding Rap guanyl-nucleotide exchange factor activity diacylglycerol binding PDZ domain binding cAMP binding cGMP binding beta-1 adrenergic receptor binding WW domain binding phosphatidic acid binding
Process(es)
MAPK cascade blood vessel development neuron migration G-protein coupled receptor signaling pathway neuropeptide signaling pathway small GTPase mediated signal transduction negative regulation of cell proliferation positive regulation of neuron projection development cAMP-mediated signaling ventricular system development forebrain neuron development microvillus assembly neuron projection development brain-derived neurotrophic factor receptor signaling pathway positive regulation of protein binding Rap protein signal transduction intracellular signal transduction nerve growth factor signaling pathway positive regulation of GTPase activity positive regulation of cAMP-mediated signaling positive regulation of protein kinase activity negative regulation of melanin biosynthetic process regulation of synaptic plasticity negative regulation of dendrite morphogenesis establishment of endothelial barrier positive regulation of ERK1 and ERK2 cascade cellular response to cAMP cellular response to cGMP adenylate cyclase-activating adrenergic receptor signaling pathway protein localization to plasma membrane establishment of endothelial intestinal barrier regulation of cell junction assembly cellular response to nerve growth factor stimulus positive regulation of cAMP-dependent protein kinase activity positive regulation of dendritic cell apoptotic process positive regulation of vasculogenesis positive regulation of neuron migration
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