NeoBiotechnologies

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Recombinant Mouse Monoclonal Antibody (rPCNA/8858)

Name: PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Recombinant Mouse Monoclonal Antibody (rPCNA\/8858)
Brand: NeoBiotechnologies
SKU: 5111-MSM11-P1ABX

View all (139) PCNA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Immunogen image

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Recombinant Mouse Monoclonal Antibody (rPCNA/8858)

Antibody Testing Data (2)

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody (5111-MSM11-P1ABX) in IHC (P)

Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858) at 2 µg/mL. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

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Product Details

5111-MSM11-P1ABX

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

rPCNA/8858

Immunogen

Recombinant full-length human PCNA protein

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive Control: HEK293 or U937 cells. Tonsil or reactive lymph node. SKBr3

Cellular Location: Nucleus. Cytoplasm.

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

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Gene Aliases: ATLD2; PCNA

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UniProt ID: (Human) P12004

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Entrez Gene ID: (Human) 5111

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Function(s)

purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Recombinant Mouse Monoclonal Antibody (rPCNA/8858)

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody (5111-MSM11-P1ABX) in IHC (P)

Product Details

5111-MSM11-P1ABX

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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