NeoBiotechnologies

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Monoclonal Antibody (PC5)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

5111-MSM3-P1ABX

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat, Yeast

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

PC5

Immunogen

Rat PCNA/Protein A fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Pol30p; Proliferating cell nuclear antigen

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Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR; POL30; YBR0811; YBR088C

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UniProt ID: (Human) P12004, (Baker's yeast) P15873, (Rat) P04961, (Mouse) P17918

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Entrez Gene ID: (Human) 5111, (Baker's yeast) 852385, (Rat) 25737, (Mouse) 18538

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Function(s)
purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding transcription factor binding
Process(es)
meiotic mismatch repair DNA replication leading strand elongation lagging strand elongation regulation of DNA replication DNA repair nucleotide-excision repair mismatch repair postreplication repair chromatin silencing at telomere cellular response to DNA damage stimulus mitotic sister chromatid cohesion chromatin silencing at silent mating-type cassette negative regulation of transcription from RNA polymerase II promoter base-excision repair, gap-filling heart development translesion synthesis epithelial cell differentiation positive regulation of deoxyribonuclease activity response to lipid response to cadmium ion cellular response to xenobiotic stimulus cellular response to UV positive regulation of DNA repair positive regulation of DNA replication regulation of catalytic activity G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell proliferation protein sumoylation replication fork processing nucleotide-excision repair, DNA incision error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication
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