Active Motif

PARP-1 N-terminal Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
PARP-1 N-terminal Polyclonal Antibody

Product Details

39559

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:50,000
View 1 publication 1 publication

ChIP assay (ChIP)

8 µL
-

ChIP-sequencing (ChIP-Seq)

-
View 1 publication 1 publication

in situ PLA (PLA)

-
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Fruit fly, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This PARP-1 N-terminal antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1 was used to generate this PARP-1 antibody.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2793257

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1

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Gene Aliases: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1

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UniProt ID: (Human) P09874

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Entrez Gene ID: (Human) 142, (Mouse) 11545

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Function(s)
DNA binding DNA ligase (ATP) activity NAD+ ADP-ribosyltransferase activity protein binding transcription factor binding zinc ion binding enzyme binding protein kinase binding identical protein binding histone deacetylase binding poly(A) RNA binding protein N-terminus binding NAD binding R-SMAD binding chromatin binding transferase activity transferase activity, transferring glycosyl groups metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding double-strand break repair via homologous recombination lagging strand elongation DNA repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion double-strand break repair transcription from RNA polymerase II promoter protein ADP-ribosylation cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway positive regulation of cardiac muscle hypertrophy protein autoprocessing protein sumoylation signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA ligation involved in DNA repair positive regulation of SMAD protein import into nucleus protein poly-ADP-ribosylation global genome nucleotide-excision repair regulation of cellular protein localization negative regulation of telomere maintenance via telomere lengthening ATP generation from poly-ADP-D-ribose positive regulation of transcription regulatory region DNA binding telomere maintenance DNA metabolic process base-excision repair transcription, DNA-templated regulation of transcription, DNA-templated regulation of growth rate behavioral response to cocaine cellular response to superoxide regulation of single strand break repair
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