Invitrogen

PABP Polyclonal Antibody

Name: PABP Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29883

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View all (12) PABP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (13)
Advanced Verification (2)

PABP Antibody in Immunocytochemistry (ICC/IF)

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PABP Antibody (PA5-29883) in ICC/IF

Immunofluorescence analysis of PABP was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PABP Rabbit Polyclonal Antibody(Product # PA5-29883) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

PABP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PABP Antibody in Immunoprecipitation (IP)

Product Image Gallery

PABP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with PABP siRNA and reduction of signal was observed in Western Blot using PABP Polyclonal Antibody (Product # PA5-29883). {KD}

Product Details

PA5-29883

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

4 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PABP. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547357

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PABP 1; PABP-1; poly A binding protein 1; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 2; poly(A)-binding protein 1; Polyadenylate-binding protein 1

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Gene Aliases: ePAB; PAB1; PABP; PABP1; PABPC1; PABPC2; PabpI; PABPL1

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UniProt ID: (Human) P11940, (Rat) Q9EPH8, (Mouse) P29341

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Entrez Gene ID: (Human) 26986, (Rat) 171350, (Mouse) 18458

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Function(s)

nucleotide binding protein binding protein C-terminus binding poly(A) binding poly(U) RNA binding translation activator activity poly(A) RNA binding mRNA binding nucleic acid binding RNA binding

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay mRNA processing RNA splicing gene silencing by RNA positive regulation of nuclear-transcribed mRNA poly(A) tail shortening positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening mRNA splicing, via spliceosome mRNA polyadenylation translational initiation regulation of mRNA stability positive regulation of viral genome replication positive regulation of translation mRNA stabilization

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PABP Polyclonal Antibody

PABP Antibody (PA5-29883) in ICC/IF

Product Details

PA5-29883

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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