Proteintech

OTX2 Polyclonal Antibody

27 References
View all (23) OTX2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

13497-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 10 publications 10 publications

Immunohistochemistry (IHC)

-
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:500
View 7 publications 7 publications

Flow Cytometry (Flow)

1:100
-

Immunoprecipitation (IP)

0.5-4.0 µg
-

ChIP assay (ChIP)

Assay-dependent
View 6 publications 6 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OTX2 Fusion Protein Ag4323 (1-297 aa encoded by BC032579)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MMSYLKQPP YAVNGLSLTT SGMDLLHPSV GYPGPWASCP AATPRKQRRE RTTFTRAQLD VLEALFAKTR YPDIFMREEV ALKINLPESR VQVWFKNRRA KCRQQQQQQQ NGGQNKVRPA KKKTSPAREV SSESGTSGQF TPPSSTSVPT IASSSAPVSI WSPASISPLS DPLSTSSSCM QRSYPMTYTQ ASGYSQGYAG STSYFGGMDC GSYLTPMHHQ LPGPGATLSP MGTNAVTSHL NQSPASLSTQ GYGASSLGFN STTDCLDYKD QTASWKLNFN ADCLDYKDQT SSWKFQVL (1-297 aa encoded by BC032579)

Target Information

OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: homeobox Otx2; Homeobox protein OTX2; Orthodenticle homolog 2; OTX

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Gene Aliases: CPHD6; E130306E05Rik; MCOPS5; OTX2

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UniProt ID: (Human) P32243

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Entrez Gene ID: (Human) 5015, (Rat) 305858, (Mouse) 18424

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding eukaryotic initiation factor 4E binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding protein heterodimerization activity
Process(es)
regulation of transcription, DNA-templated multicellular organism development transcription from RNA polymerase II promoter protein complex assembly axon guidance regulation of smoothened signaling pathway forebrain development midbrain development positive regulation of embryonic development regulation of fibroblast growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter dopaminergic neuron differentiation primitive streak formation positive regulation of gastrulation cell fate specification regulation of transcription from RNA polymerase II promoter central nervous system development endoderm development organ morphogenesis anterior/posterior pattern specification dorsal/ventral pattern formation metencephalon development cell differentiation neuron differentiation somite rostral/caudal axis specification inner ear morphogenesis eye photoreceptor cell fate commitment cell fate commitment neuron fate commitment neuron fate determination diencephalon morphogenesis anatomical structure development
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