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  • OTUB1 Antibodies

OriGene

OTUB1 Monoclonal Antibody (OTI5F7), TrueMAB™

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Datasheet
Protocols
Questions & Answers

Cite OTUB1 Monoclonal Antibody (OTI5F7), TrueMAB™

  • Antibody Testing Data (11)
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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OTUB1 Antibody (CF505204) in IHC (P)

Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505204) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
OTUB1 Antibody in Western Blot (WB)
OTUB1 Antibody in Western Blot (WB)
OTUB1 Monoclonal Antibody (OTI5F7), TrueMAB™

Product Details

CF505204

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI5F7

Immunogen

Full length human recombinant protein of human OTUB1 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated
  • Biotin 
  • Request custom conjugation

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

The product of this gene is a member of the OTU superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Deubiquitinating enzyme OTUB1; FLJ20113; FLJ40710; hOTU1; MGC111158; MGC4584; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; Otubain-1; Ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; Ubiquitin-specific-processing protease OTUB1

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Gene Aliases: HSPC263; OTB1; OTU1; OTUB1

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UniProt ID: (Human) Q96FW1

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Entrez Gene ID: (Human) 55611

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Function(s)
thiol-dependent ubiquitin-specific protease activity protein binding NEDD8-specific protease activity ubiquitin protein ligase binding ubiquitin binding
Process(es)
adaptive immune response DNA repair cellular response to DNA damage stimulus protein K48-linked deubiquitination cellular response to interleukin-1 negative regulation of histone H2A K63-linked ubiquitination negative regulation of double-strand break repair
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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