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  • Nucleolin Antibodies

Invitrogen

Nucleolin Monoclonal Antibody (4E2)

Advanced Verification
5 References
View all (50) Nucleolin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Nucleolin Monoclonal Antibody (4E2)

  • Antibody Testing Data (5)
  • Advanced Verification (3)
Nucleolin Antibody in Immunocytochemistry (ICC/IF)
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Nucleolin Antibody in Immunocytochemistry (ICC/IF)
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Nucleolin Antibody (MA1-20800) in ICC/IF

Immunofluorescence analysis of Nucleolin was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Nucleolin Monoclonal Antibody (4E2) (Product # MA1-20800) at 5 microgram/mL concentration in 0.1% BSA, incubated at 4 degree celsius over... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Nucleolin Antibody in Immunocytochemistry (ICC/IF)
Nucleolin Antibody in Immunohistochemistry (IHC)
Nucleolin Antibody in Western Blot (WB)
Nucleolin Antibody in Western Blot (WB)
Nucleolin Antibody in Western Blot (WB)
Nucleolin Antibody
Nucleolin Antibody
Nucleolin Antibody
Nucleolin Monoclonal Antibody (4E2)

Product Details

MA1-20800

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1-10 µg/mL
-

Immunocytochemistry (ICC/IF)

1-10 µg/mL
View 4 publications 4 publications

ChIP assay (ChIP)

Assay-dependent
-

in situ PLA (PLA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Human

Published species

Bovine, Human, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E2

Immunogen

Human nucleolin protein from Raji cell extract.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 50% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560407

Product Specific Information

Recommended positive controls: Raji cell extract.

This antibody does not react with mouse protein.

Target Information

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: FLJ45706; FLJ59041; nucl antibody; Nucleolin; Protein C23

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Gene Aliases: BOS_2214; C23; NCL

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UniProt ID: (Human) P19338

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Entrez Gene ID: (Bovine) 497013, (Human) 4691

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Function(s)
nucleotide binding single-stranded DNA binding RNA binding calcium ion binding protein binding protein C-terminus binding selenocysteine insertion sequence binding rRNA primary transcript binding telomeric DNA binding histone binding identical protein binding laminin binding poly(A) RNA binding ErbB-4 class receptor binding
Process(es)
angiogenesis endocytosis spermatogenesis positive regulation of tumor necrosis factor production negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter cellular response to lipopolysaccharide liver regeneration positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter regulation of rRNA processing positive regulation of interleukin-6 secretion
It has to be done as per old AB suggested Products section.
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