Invitrogen

NuMA Polyclonal Antibody

Name: NuMA Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-32451

Advanced Verification

1 Published Figure

1 Reference

View all (31) NuMA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

NuMA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 25

NuMA Antibody (PA1-32451) in ICC/IF

Immunofluorescence analysis of NuMA was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NuMA Polyclonal Antibody (Product # PA1-32451) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More

NuMA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

NuMA Antibody — Antibody specificity was demonstrated by detection of differential localisations of the target across mitosis. Differential localisation of NuMA was tested in HeLa cells in different phases of cell cycle, and was observed to be nuclear in the interphase cells, localised to the spindle poles in metaphasic cells, and loss of this upon dissolution of the spindle apparatus with progression through Anaphase, using NuMA Polyclonal Antibody (Product # PA1-32451) in immunofluorescence. {RE}

Immunogen image

Immunogen Image NuMA Polyclonal Antibody

Product Details

PA1-32451

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:500

Immunoprecipitation (IP)

1:10-1:500

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2154615

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein

View more View less

Gene Aliases: 6720401E04Rik; AA764025; AL022610; AU014979; NMP-22; NMP22; NUMA; NUMA1

View more View less

UniProt ID: (Human) Q14980, (Mouse) E9Q7G0

View more View less

Entrez Gene ID: (Human) 4926, (Rat) 308870, (Mouse) 101706

View more View less

Function(s)

structural molecule activity protein binding microtubule binding phosphorelay sensor kinase activity tubulin binding

Process(es)

establishment of mitotic spindle orientation nucleus organization mitotic nuclear division cell division meiotic cell cycle lung epithelial cell differentiation phosphorelay signal transduction system signal transduction by protein phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NuMA Polyclonal Antibody

NuMA Antibody (PA1-32451) in ICC/IF

Product Details

PA1-32451

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!