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NeoBiotechnologies

Neurofilament (NF-L) (Neuronal Marker) Monoclonal Antibody (NFL/736)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Neurofilament (NF-L) (Neuronal Marker) Monoclonal Antibody (NFL/736)

  • Antibody Testing Data (2)
Neurofilament (NF-L) (Neuronal Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Neurofilament (NF-L) (Neuronal Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Neurofilament (NF-L) (Neuronal Marker) Antibody (4747-MSM2-P0) in IHC (P)

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Neurofilament (NF-L) (Neuronal Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neurofilament (NF-L) (Neuronal Marker) Antibody in Flow Cytometry (Flow)

Product Details

4747-MSM2-P0

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

0.25-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

2-4 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Bovine, Chicken, Human, Pig, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

NFL/736

Immunogen

Recombinant human NEFL protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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Gene Aliases: BOS_9100; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Bovine) P02548, (Human) P07196, (Rat) P19527

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Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Rat) 83613

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Function(s)
structural constituent of cytoskeleton protein C-terminus binding identical protein binding Ras guanyl-nucleotide exchange factor activity protein binding protein domain specific binding protein binding, bridging phospholipase binding protein heterodimerization activity
Process(es)
microtubule cytoskeleton organization anterograde axonal transport retrograde axonal transport peripheral nervous system axon regeneration axonal transport of mitochondrion regulation of axon diameter neurofilament bundle assembly locomotion negative regulation of neuron apoptotic process intermediate filament organization intermediate filament bundle assembly neuron projection morphogenesis positive regulation of axonogenesis neuromuscular process controlling balance neurofilament cytoskeleton organization MAPK cascade response to toxic substance spinal cord development hippocampus development cerebral cortex development response to peptide hormone positive regulation of GTPase activity intermediate filament polymerization or depolymerization protein polymerization response to corticosterone response to sodium arsenite response to acrylamide response to organic substance response to organonitrogen compound
It has to be done as per old AB suggested Products section.
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