Invitrogen

NR0B1 Polyclonal Antibody

Name: NR0B1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-18716

View all (21) NR0B1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

NR0B1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

NR0B1 Antibody (PA5-18716) in ICC/IF

Immunocytochemistry analysis of NR0B1 using NR0B1 Polyclonal Antibody (Product # PA5-18716) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

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Immunogen image

Immunogen Image NR0B1 Polyclonal Antibody

Product Details

PA5-18716

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

Immunocytochemistry (ICC/IF)

10 µg/mL

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (CGEDHPQQGSTLY) corresponding to the internal amino acids of NR0B1 (aa 202-215).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10988362

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DAX 1; DSS-AHC critical region on the X chromosome protein 1; nuclear hormone receptor; Nuclear receptor DAX-1; Nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1; R0B1

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Gene Aliases: AHC; AHCH; AHX; DAX-1; DAX1; DSS; GTD; HHG; NR0B1; NROB1; SRXY2

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UniProt ID: (Human) P51843

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Entrez Gene ID: (Human) 190

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Function(s)

DNA binding steroid hormone receptor activity transcription corepressor activity RNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding protein domain specific binding DNA hairpin binding steroid hormone receptor binding protein homodimerization activity sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter steroid biosynthetic process spermatogenesis protein localization gonad development male gonad development hypothalamus development pituitary gland development male sex determination adrenal gland development intracellular receptor signaling pathway negative regulation of intracellular steroid hormone receptor signaling pathway Leydig cell differentiation response to immobilization stress steroid hormone mediated signaling pathway negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of cell differentiation negative regulation of transcription, DNA-templated Sertoli cell differentiation

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NR0B1 Polyclonal Antibody

NR0B1 Antibody (PA5-18716) in ICC/IF

Product Details

PA5-18716

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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