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  • Primary Antibodies ›
  • NQO1 Antibodies

Invitrogen

NQO1 Monoclonal Antibody (A180)

Advanced Verification
5 Published Figures
10 References
View all (38) NQO1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NQO1 Monoclonal Antibody (A180)

  • Antibody Testing Data (3)
  • Published Figures (5)
  • Advanced Verification (2)
NQO1 Antibody in Immunocytochemistry (ICC/IF)
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NQO1 Antibody in Immunocytochemistry (ICC/IF)
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NQO1 Antibody (39-3700) in ICC/IF

Immunofluorescence analysis of NQO1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NQO1 (A180) Mouse Monoclonal Antibody (Product # 39-3700) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NQO1 Antibody in Immunocytochemistry (ICC/IF)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody in Western Blot (WB)
NQO1 Antibody
NQO1 Antibody

Product Details

39-3700

Applications
Tested Dilution
Publications

Western Blot (WB)

2-4 µg/mL
View 9 publications 9 publications

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A180

Immunogen

Recombinant human NQO1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533410

Target Information

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Azoreductase; Diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase 4; diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NQ01; Phylloquinone reductase; QR1; Quinone reductase 1

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Gene Aliases: DHQU; DIA4; DTD; NMOR1; NMORI; NQO1; QR1

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UniProt ID: (Human) P15559, (Rat) P05982

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Entrez Gene ID: (Dog) 610935, (Human) 1728, (Rat) 24314

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Function(s)
NAD(P)H dehydrogenase (quinone) activity cytochrome-b5 reductase activity, acting on NAD(P)H superoxide dismutase activity protein binding identical protein binding poly(A) RNA binding
Process(es)
regulation of cellular amino acid metabolic process xenobiotic metabolic process nitric oxide biosynthetic process synaptic transmission, cholinergic aging response to nutrient response to toxic substance removal of superoxide radicals response to estradiol negative regulation of catalytic activity positive regulation of neuron apoptotic process response to ethanol oxidation-reduction process superoxide metabolic process response to oxidative stress response to organic cyclic compound
It has to be done as per old AB suggested Products section.
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