Invitrogen

NEDD4L Recombinant Rabbit Monoclonal Antibody (3W9J2)

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Datasheet
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Datasheet
Protocols
Questions & Answers
NEDD4L Recombinant Rabbit Monoclonal Antibody (3W9J2)

Product Details

MA5-42908

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3W9J2

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 876-975 of human NEDD4L (Q96PU5).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.75 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912049

Product Specific Information

Positive test controls include: HeLa, K-562.

Immunogen sequence: NHPVIQWFWK AVLLMDAEKR IRLLQFVTGT SRVPMNGFAE LYGSNGPQLF TIEQWGSPEK LPRAHTCFNR LDLPPYETFE DLREKLLMAV ENAQGFEGVD

Target Information

NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase NEDD4-like; FLJ33870; HECT-type E3 ubiquitin transferase NED4L; Nedd4-2; NEDD4.2; ubiquitin-protein ligase Rsp5

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Gene Aliases: hNEDD4-2; KIAA0439; NEDD4-2; NEDD4.2; NEDD4L; NEDL3; RSP5

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UniProt ID: (Human) Q96PU5

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Entrez Gene ID: (Human) 23327

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Function(s)
ubiquitin-protein transferase activity protein binding potassium channel regulator activity ligase activity sodium channel regulator activity potassium channel inhibitor activity sodium channel inhibitor activity ion channel binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination regulation of membrane depolarization protein monoubiquitination sodium ion transport cellular sodium ion homeostasis excretion response to metal ion protein ubiquitination viral life cycle water homeostasis ion transmembrane transport regulation of ion transmembrane transport regulation of protein catabolic process regulation of membrane potential protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of protein catabolic process positive regulation of endocytosis regulation of membrane repolarization protein K48-linked ubiquitination ventricular cardiac muscle cell action potential regulation of potassium ion transmembrane transporter activity negative regulation of potassium ion transmembrane transporter activity negative regulation of potassium ion transmembrane transport negative regulation of sodium ion transmembrane transport positive regulation of dendrite extension negative regulation of protein localization to cell surface negative regulation of sodium ion transmembrane transporter activity positive regulation of caveolin-mediated endocytosis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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