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  • NCK1 Antibodies

Invitrogen

NCK1 Polyclonal Antibody

View all (13) NCK1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite NCK1 Polyclonal Antibody

  • Antibody Testing Data (1)
NCK1 Antibody in Western Blot (WB)
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NCK1 Antibody in Western Blot (WB)
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NCK1 Antibody (PA5-87556) in WB

Western blot analysis of NCK1 in mouse brain. Samples were incubated with NCK1 Polyclonal antibody (Product # PA5-87556) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NCK1 Antibody in Western Blot (WB)
NCK1 Polyclonal Antibody

Product Details

PA5-87556

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 58-377 of human NCK1 (NP_0061441)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.69 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804250

Product Specific Information

Immunogen sequence: RKNSARKASI VKNLKDTLGI GKVKRKPSVP DSASPADDSF VDPGERLYDL NMPAYVKFNY MAEREDELSL IKGTKVIVME KCSDGWWRGS YNGQVGWFPS NYVTEEGDSP LGDHVGSLSE KLAAVVNNLN TGQVLHVVQA LYPFSSSNDE ELNFEKGDVM DVIEKPENDP EWWKCRKING MVGLVPKNYV TVMQNNPLTS GLEPSPPQCD YIRPSLTGKF AGNPWYYGKV TRHQAEMALN ERGHEGDFLI RDSESSPNDF SVSLKAQGKN KHFKVQLKET VYCIGQRKFS TMEELVEHYK KAPIFTSEQG EKLYLVKHLS; Positive Samples: Mouse brain; Cellular Location: Cytoplasm, Endoplasmic reticulum, Nucleus

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytoplasmic protein Nck1; MGC12668; NCK adapter protein 1; NCK adaptor protein 1; Nck-1; SH2/SH3 adapter protein Nck1

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Gene Aliases: 6330586M15Rik; D230010O13Rik; Nck; Nck-1; Nck1

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UniProt ID: (Mouse) Q99M51

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Entrez Gene ID: (Mouse) 17973

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Function(s)
protein kinase inhibitor activity receptor binding protein binding protein kinase regulator activity protein domain specific binding receptor tyrosine kinase binding eukaryotic initiation factor eIF2 binding
Process(es)
regulation of translation negative regulation of protein kinase activity substrate-dependent cell migration, cell extension actin filament organization cell migration lamellipodium assembly positive regulation of actin filament polymerization negative regulation of peptidyl-serine phosphorylation positive regulation of translation in response to endoplasmic reticulum stress positive regulation of T cell proliferation T cell activation positive regulation of transcription from RNA polymerase II promoter response to other organism negative regulation of cell death peptidyl-serine dephosphorylation positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of cap-dependent translational initiation positive regulation of cap-independent translational initiation negative regulation of PERK-mediated unfolded protein response negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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