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  • Primary Antibodies ›
  • p47phox Antibodies

Proteintech

NCF1 Polyclonal Antibody

3 References
View all (23) p47phox antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NCF1 Polyclonal Antibody

  • Antibody Testing Data (3)
NCF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NCF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NCF1 Antibody (28187-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28187-1-AP (p47 phox antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NCF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NCF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NCF1 Antibody in Western Blot (WB)

Product Details

28187-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:600
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NCF1 Fusion Protein Ag28089

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

300 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; SH3 and PX domain-containing protein 1A

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Gene Aliases: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

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UniProt ID: (Human) P14598

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Entrez Gene ID: (Human) 653361

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Function(s)
protein binding electron carrier activity superoxide-generating NADPH oxidase activity superoxide-generating NADPH oxidase activator activity SH3 domain binding phosphatidylinositol binding phosphatidylinositol-3,4-bisphosphate binding
Process(es)
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent protein targeting to membrane superoxide metabolic process cellular defense response superoxide anion generation positive regulation of catalytic activity innate immune response cell redox homeostasis respiratory burst vascular endothelial growth factor receptor signaling pathway oxidation-reduction process
It has to be done as per old AB suggested Products section.
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