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  • Myosin Skeletal Muscle Antibodies

Invitrogen

Myosin Skeletal Muscle Polyclonal Antibody

Advanced Verification
1 Published Figure
4 References
View all (6) Myosin Skeletal Muscle antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Myosin Skeletal Muscle Polyclonal Antibody

  • Antibody Testing Data (4)
  • Published Figures (1)
  • Advanced Verification (2)
Myosin Skeletal Muscle Antibody in Immunocytochemistry (ICC/IF)
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Myosin Skeletal Muscle Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 7

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Myosin Skeletal Muscle Antibody (PA5-31466) in ICC/IF

Immunofluorescence analysis of Myosin Skeletal Muscle was performed using C2C12 and C2C12 cells differentiated to myotubes (2% Horse serum for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Myosin Skeletal Muscle Rabbit Polyclonal Antibody (Product... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Myosin Skeletal Muscle Antibody in Immunocytochemistry (ICC/IF)
Myosin Skeletal Muscle Antibody in Western Blot (WB)
Myosin Skeletal Muscle Antibody in Western Blot (WB)
Myosin Skeletal Muscle Antibody in Western Blot (WB)
Myosin Skeletal Muscle Antibody in Western Blot (WB)
Myosin Skeletal Muscle Antibody
Myosin Skeletal Muscle Antibody
Myosin Skeletal Muscle Polyclonal Antibody

Product Details

PA5-31466

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

5 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MYH1. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2548939

Product Specific Information

Recommended positive controls: RD, mouse heart.

Predicted reactivity: Mouse (98%), Rat (97%), Xenopus laevis (81%), Dog (98%), Pig (99%), Rabbit (97%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2A myosin heavy chain; 2B myosin heavy chain; epididymis luminal protein 71; fast 2a myosin heavy chain; inclusion body myopathy 3, autosomal dominant; MGC133384; myh4; MyHC-2a; MyHC-2b; MyHC-2x; MyHC-IIa; MyHC-IIb; MyHC-IIx/d; Myosin heavy chain 1; Myosin heavy chain 2; Myosin heavy chain 2a; Myosin heavy chain 2b; Myosin heavy chain 2x; Myosin heavy chain 4; Myosin heavy chain IIa; Myosin heavy chain IIb; Myosin heavy chain IIx/d; myosin heavy chain type IIb; myosin heavy chain type IIx; Myosin heavy chain, skeletal muscle, adult 1; Myosin heavy chain, skeletal muscle, adult 2; Myosin heavy chain, skeletal muscle, fetal; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult; myosin, heavy polypeptide 4, skeletal muscle; Myosin-1; Myosin-2; Myosin-4; skeletal muscle myosin heavy chain II-a; type 2X myosin heavy chain; type IIA myosin heavy chain

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Gene Aliases: A530084A17Rik; AI506973; HEL71; IBM3; IId; IId/x; MdMs; MHC-2X/D; MHC2A; MHC2B; MHC2X/D; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2; MYH2A; MYH2B; MYH4; MYHa; MYHas8; MYHC; MyHC-2A; MyHC-2B; MyHC-2x; MyHC-2X/D; MyHC-IIA; MyHC-IIb; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f1; Myhs-f2; MYHSA1; MYHSA2; Myhsf; Myhsf1; Myhsf2; MYPOP

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UniProt ID: (Human) P12882, (Human) Q9UKX2, (Human) Q9Y623, (Mouse) Q5SX40, (Mouse) G3UW82, (Mouse) Q5SX39, (Rat) Q29RW1

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Entrez Gene ID: (Human) 4619, (Human) 4620, (Human) 4622, (Mouse) 17879, (Rat) 287408, (Rat) 691644, (Mouse) 17882, (Mouse) 17884, (Rat) 360543

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Function(s)
motor activity actin binding protein binding calmodulin binding ATP binding microfilament motor activity double-stranded RNA binding ATPase activity nucleotide binding molecular_function
Process(es)
plasma membrane repair muscle contraction metabolic process response to activity myosin filament assembly actin-mediated cell contraction actin filament-based movement muscle filament sliding ATP metabolic process Fc-gamma receptor signaling pathway involved in phagocytosis response to gravity response to muscle activity response to muscle stretch muscle structure development striated muscle contraction
It has to be done as per old AB suggested Products section.
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