Invitrogen

Mouse anti-Canine IgE Secondary Antibody

Name: Mouse anti-Canine IgE Secondary Antibody
Brand: Invitrogen
SKU: SA5-10299

Datasheet

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Questions & Answers

Datasheet

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Questions & Answers

Antibody Testing Data (1)

Canine IgE Secondary Antibody in ELISA (ELISA)

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Canine IgE Secondary Antibody (SA5-10299) in ELISA

ELISA analysis was performed using Dog IgE antigen (500 ng/well, 60 min). Samples were blocked for 60 min with 1% BSA, incubated with Mouse anti-Dog IgE (60 min), and followed by HRP Goat anti-Dog IgE (Product # SA5-10299) for 60 min. Development was done with TMB substrate (10 min) and the reaction was stopped with 0.3M H2SO4. Results were read on a microplate reader at OD450.

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Product Details

SA5-10299

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Dog

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

E62

Immunogen

Canine IgE

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.8 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2868347

Target

IgE

Antibody Form

Whole Antibody

Target Information

Anti-Canine secondary antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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