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  • Isotype Controls ›
  • Mouse IgG2b kappa Isotype Controls

Invitrogen

Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™

1 Published Figure
View all Mouse IgG2b kappa Isotype Controls

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Cite Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™

  • Testing Data (1)
  • Published Figures (1)
Mouse IgG2b kappa Isotype Control in Flow Cytometry (Flow)
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Mouse IgG2b kappa Isotype Control in Flow Cytometry (Flow)
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FIGURE: 1 / 2

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Mouse IgG2b kappa Isotype Control (11-4732-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45 eFluor® 450 (Product # 48-9459-42) and Mouse IgG2b K Isotype Control FITC (Product # 11-4732-42) (left) or Anti-Human CD133 FITC (right). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse IgG2b kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG2b kappa Isotype Control in Flow Cytometry (Flow)

Product Details

11-4732-42

Applications
Tested Dilution

Immunohistochemistry (IHC)

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

5 µL (1 µg)/test

Control (Ctrl)

Assay-Dependent
Product Specifications

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Isotype Control

Clone

eBMG2b

Conjugate

FITC FITC FITC
  • Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 532 
  • Alexa Fluor 647 
  • Alexa Fluor 660 
  • Alexa Fluor 700 
  • APC 
  • APC-eFluor 780
  • Biotin 
  • Brilliant UV 395
  • Brilliant UV 496
  • Brilliant UV 615
  • Brilliant UV 661
  • Brilliant UV 737
  • Brilliant UV 805
  • Brilliant Violet 421
  • Brilliant Violet 480
  • Brilliant Violet 605
  • Brilliant Violet 650
  • Brilliant Violet 711
  • Brilliant Violet 786
  • eFluor 450
  • eFluor 506
  • eFluor 570
  • eFluor 615
  • eFluor 660
  • Functional Grade
  • PE 
  • PE-Cyanine5
  • PE-Cyanine5.5
  • PE-Cyanine7
  • PE-eFluor 610
  • PerCP-Cyanine5.5
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Super Bright 645
  • Super Bright 702
  • Super Bright 780
  • Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598648

Product Specific Information

Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.

Applications Reported:This mouse IgG2b isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by intracellular flow cytometric analysis and can be used at test size: 5 µL (1 µg)/test per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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