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  • Isotype Controls ›
  • Mouse IgG1 kappa Isotype Controls

Invitrogen

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

View all Mouse IgG1 kappa Isotype Controls

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Cite Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

  • Testing Data (26)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
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Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
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FIGURE: 1 / 26

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Mouse IgG1 kappa Isotype Control (12-4714-82) in Flow

BALB/c mouse bone marrow cells were Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or 0.25 µg of CD64 Monoclonal Antibody, PE (right). All cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)

Product Details

12-4714-82

Applications
Tested Dilution

Flow Cytometry (Flow)

Assay-Dependent

Control (Ctrl)

Assay-Dependent
Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Isotype Control

Clone

P3.6.2.8.1

Conjugate

PE PE PE
  • Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 532 
  • Alexa Fluor 561
  • Alexa Fluor 647 
  • Alexa Fluor 660 
  • Alexa Fluor 700 
  • APC 
  • APC-eFluor 780
  • Biotin 
  • Brilliant UV 395
  • Brilliant UV 496
  • Brilliant UV 563
  • Brilliant UV 615
  • Brilliant UV 661
  • Brilliant UV 737
  • Brilliant UV 805
  • Brilliant Violet 421
  • Brilliant Violet 480
  • Brilliant Violet 605
  • Brilliant Violet 650
  • Brilliant Violet 711
  • Brilliant Violet 786
  • eFluor 450
  • eFluor 506
  • eFluor 570
  • eFluor 615
  • eFluor 660
  • FITC
  • Functional Grade
  • Pacific Orange
  • PE-Cyanine5
  • PE-Cyanine5.5
  • PE-Cyanine7
  • PE-eFluor 610
  • PerCP-Cyanine5.5
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Super Bright 645
  • Super Bright 702
  • Super Bright 780
  • Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_470060

Product Specific Information

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.

Applications Reported: PE Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.

Applications Tested: This Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis. Use isotype control at the same concentration as experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis. This can be used at test size: 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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