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  • Primary Antibodies ›
  • H3K4me1 Antibodies
Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Invitrogen

H3K4me1 Recombinant Superclonal™ Antibody, ChIP-Verified

Advanced Verification
5 References
View all (17) H3K4me1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite H3K4me1 Recombinant Superclonal™ Antibody, ChIP-Verified

  • Antibody Testing Data (9)
  • Advanced Verification (3)
H3K4me1 Antibody in Immunocytochemistry (ICC/IF)
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H3K4me1 Antibody in Immunocytochemistry (ICC/IF)
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H3K4me1 Antibody (710795) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized Hela cells for detection of Histone H3K4me1 using Anti-Histone H3K4me1 Recombinant Rabbit Superclonal™ Antibody (Product # 710795, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H3K4me1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mounta... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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H3K4me1 Antibody in Immunocytochemistry (ICC/IF)
H3K4me1 Antibody in Western Blot (WB)
H3K4me1 Antibody in Western Blot (WB)
H3K4me1 Antibody in Western Blot (WB)
H3K4me1 Antibody in ChIP Assay (ChIP)
H3K4me1 Antibody in ChIP Assay (ChIP)
H3K4me1 Antibody in ChIP-Sequencing (ChIP-seq)
H3K4me1 Antibody in Peptide array (ARRAY)
H3K4me1 Antibody in CUT&RUN (C&R)
H3K4me1 Antibody
H3K4me1 Antibody
H3K4me1 Antibody
H3K4me1 Recombinant Superclonal™ Antibody, ChIP-Verified

Product Details

710795

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ChIP assay (ChIP)

1-5 µg
View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

3 µg
-

Peptide Array (Array)

0.25 µg/mL
-

CUT&RUN (C&R)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Methylated peptide (Lys4) corresponding to human Histone H3 (aa 2-11)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532764

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K4; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K4; H3K4me; H3Lys4me; H3Lys4me1; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K4me1; methyl Histone 3; methyl Histone H3; Methyl-H3 K4; Methyl-H3 Lys4; Methyl-Histone H3 K4; Methyl-Histone H3 Lys4; Mono-methyl H3; Mono-methyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)
DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion
Process(es)
chromatin silencing at rDNA nucleosome assembly blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing telomere organization cell-cell adhesion xenophagy
It has to be done as per old AB suggested Products section.
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