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  • Myotilin Antibodies

OriGene

MYOT Monoclonal Antibody (OTI8A7), TrueMAB™

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite MYOT Monoclonal Antibody (OTI8A7), TrueMAB™

  • Antibody Testing Data (1)
MYOT Antibody in Western Blot (WB)
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MYOT Antibody in Western Blot (WB)
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MYOT Antibody (CF809549) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYOT (RC202797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MYOT. (1. Positive lysates LY402026 (100 µg) and LC402026 (20 µg) can be purchased separately from OriGene. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MYOT Antibody in Western Blot (WB)
MYOT Monoclonal Antibody (OTI8A7), TrueMAB™

Product Details

CF809549

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI8A7

Immunogen

Full length human recombinant protein of human MYOT produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 57 kDa cytoskeletal protein; Myofibrillar titin-like Ig domains protein; Myotilin; titan-like protein; Titin immunoglobulin domain protein; titin immunoglobulin domain protein (myotilin)

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Gene Aliases: 5530402I04Rik; LGMD1; LGMD1A; MFM3; Myo; MYOT; TTID; TTOD

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UniProt ID: (Human) Q9UBF9, (Mouse) Q9JIF9

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Entrez Gene ID: (Human) 9499, (Mouse) 58916, (Rat) 291605

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Function(s)
actin binding protein binding structural constituent of muscle alpha-actinin binding molecular_function
Process(es)
biological_process muscle contraction
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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