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  • Primary Antibodies ›
  • MTHFD1L Antibodies

Invitrogen

MTHFD1L Polyclonal Antibody

View all (11) MTHFD1L antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite MTHFD1L Polyclonal Antibody

  • Antibody Testing Data (4)
MTHFD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MTHFD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MTHFD1L Antibody (PA5-96880) in IHC (P)

Immunohistochemistry analysis of MTHFD1L in paraffin-embedded mouse spinal cord. Samples were incubated with MTHFD1L Polyclonal antibody (Product # PA5-96880) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MTHFD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MTHFD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MTHFD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MTHFD1L Antibody in Western Blot (WB)
MTHFD1L Polyclonal Antibody

Product Details

PA5-96880

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 759-978 of human MTHFD1L (NP_0562552)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.68 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2808682

Product Specific Information

Immunogen sequence: GVPLKKEYTE ENIQLVADGC CNLQKQIQIT QLFGVPVVVA LNVFKTDTRA EIDLVCELAK RAGAFDAVPC YHWSVGGKGS VDLARAVREA ASKRSRFQFL YDVQVPIVDK IRTIAQAVYG AKDIELSPEA QAKIDRYTQQ GFGNLPICMA KTHLSLSHQP DKKGVPRDFI LPISDVRASI GAGFIYPLVG TMSTMPGLPT RPCFYDIDLD TETEQVKGLF; Positive Samples: A-549, placenta, Mouse brain, Mouse heart, Rat brain; Cellular Location: Mitochondrion

Target Information

One-carbon substituted forms of tetrahydrofolate (THF) are involved in the de novo synthesis of purines and thymidylate and support cellular methylation reactions through the regeneration of methionine from homocysteine. MTHFD1L is an enzyme involved in THF synthesis in mitochondria (Christensen et al., 2005 [PubMed 15611115]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 10-formyl-THF synthetase; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; Monofunctional C1-tetrahydrofolate synthase, mitochondrial

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Gene Aliases: 2410004L15Rik; AI647056; dJ292B18.2; FTHFSDC1; MTC1THFS; MTHFD1L

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UniProt ID: (Human) Q6UB35, (Mouse) Q3V3R1

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Entrez Gene ID: (Human) 25902, (Rat) 361472, (Mouse) 270685

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Function(s)
formate-tetrahydrofolate ligase activity methenyltetrahydrofolate cyclohydrolase activity methylenetetrahydrofolate dehydrogenase (NADP+) activity ATP binding protein homodimerization activity molecular_function nucleotide binding catalytic activity ligase activity
Process(es)
one-carbon metabolic process folic acid-containing compound metabolic process biological_process folic acid-containing compound biosynthetic process formate metabolic process tetrahydrofolate metabolic process oxidation-reduction process 10-formyltetrahydrofolate biosynthetic process neural tube closure tetrahydrofolate interconversion folic acid metabolic process embryonic neurocranium morphogenesis embryonic viscerocranium morphogenesis
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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